Comparative phosphoproteomic analysis of developing maize seeds suggests a pivotal role for enolase in promoting starch synthesis
Comparative phosphoproteomic analysis of developing maize seeds suggests a pivotal role for enolase in promoting starch synthesis
复制标题
发育中的玉米种子的比较磷酸蛋白质组学分析表明烯醇酶在促进淀粉合成中的关键作用
DOI：
10.1016/j.plantsci.2019.110243
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发表时间：
2019-12-01
期刊：
影响因子：
5.2
通讯作者：
Ren, Dongtao
中科院分区：
文献类型：
--
作者：
Cao, Hanwei;Zhou, Yuwei;Ren, Dongtao
玉米种子是全世界淀粉的主要来源，也是研究淀粉合成的理想模型。种子淀粉含量是一种典型的数量表型，许多研究表明糖酵解酶参与了淀粉合成的调控，但其调控机制尚不清楚。在这里，我们提出了一个比较磷酸化蛋白质组学研究的三个玉米自交系具有不同的种子淀粉含量。结果表明，玉米种子发育过程中有62种蛋白质和63种磷蛋白的丰度受到调控。利用糖酵解和淀粉合成相关的17种酶的动力学，构建了淀粉合成的磷酸化调控网络。这表明玉米种子中淀粉合成和糖酵解利用来自山梨糖醇和蔗糖的相同己糖磷酸库作为碳源，并且ZmENO1的磷酸化有助于增加淀粉含量，因为它与不同发育阶段和不同品系的种子淀粉含量正相关，磷酸模拟突变体（ZmENO1（S43D））破坏了其在糖酵解中至关重要的酶活性。本研究结果为玉米种子淀粉合成的调控过程提供了新的视角，并可用于高淀粉含量玉米育种。
Maize (Zea mays) seeds are the major source of starch all over the world and the excellent model for researching starch synthesis. Seed starch content is a typical quantitative phenotype and many reports revealed that the glycolytic enzymes are involved in regulating starch synthesis, however the regulatory mechanism is still unclear. Here, we present a comparative phosphoproteomic study of three maize inbred lines with different seed starch content. It reveals that abundances of 62 proteins and 63 phosphoproteins were regulated during maize seed development. Dynamics of 17 enzymes related to glycolysis and starch synthesis were used to construct a phosphorylation regulatory network of starch synthesis. It shows that starch synthesis and glycolysis in maize seeds utilize the same hexose phosphates pool coming from sorbitol and sucrose as carbon source, and phosphorylation of ZmENO1 are suggested to contribute to increase starch content, because it is positively related to seed starch content in different developmental stages and different lines, and the phosphor-mimic mutant (ZmENO1(S43D)) damaged its enzyme activity which is vital in glycolysis. Our results provide a new sight into regulatory process of seed starch synthesis and can be used in maize breeding for high starch content.