A leucine-rich repeat receptor-like kinase, OsSTLK, modulates salt tolerance in rice
A leucine-rich repeat receptor-like kinase, OsSTLK, modulates salt tolerance in rice
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富含亮氨酸的重复受体样激酶 OsSTLK 可调节水稻的耐盐性
DOI：
10.1016/j.plantsci.2020.110465
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发表时间：
2020-07-01
期刊：
影响因子：
5.2
通讯作者：
Du, Changqing
中科院分区：
文献类型：
--
作者：
Lin, Faming;Li, Shen;Du, Changqing
富含亮氨酸的重复受体样激酶（LRR-RLKs）与植物的非生物胁迫反应有着广泛的联系。然而，大多数lrr - rlk的功能尚未得到很好的定义。本研究鉴定了水稻LRR-RLK新成员，并将其命名为OsSTLK （Oryza sativa L. salt - tolerance LRR-RLK）。转录本分析表明，盐胁迫诱导OsSTLK在水稻茎部和根系中表现出明显的时间依赖性和剂量依赖性。表型观察表明，OsSTLK过表达降低了盐敏感性，提高了盐胁迫耐受性。进一步的生理分析表明，OsSTLK过表达通过上调活性氧清除活性和改变气孔模式，显著降低盐胁迫条件下的电解质泄漏、丙二醛（MDA）含量和活性氧（ROS）积累。此外，与WT对照相比，过表达osstlk的水稻植株Na+/K+比值和MAPK磷酸化水平也有所降低。综上所述，我们的研究结果表明，OsSTLK可能通过调节ROS清除系统、Na+/K+比值和MAPK信号通路，作为盐胁迫耐受性的重要正调控因子。
Leucine-rich repeat receptor-like kinases (LRR-RLKs) have been widely associated with plant abiotic stress responses. However, the functions of the majority of LRR-RLKs has not been well defined. Here, we identified a novel rice LRR-RLK member involved in salt tolerance and designated as OsSTLK (Oryza sativa L. Salt-Tolerance LRR-RLK). Transcript analysis showed that OsSTLK was significantly induced in response to salt stress in rice shoot and root in a time and dosage-dependent fashion. Phenotypic observations indicated that OsSTLK overexpression exhibited reduced salt sensitivity, and improved salt stress tolerance. Further physiological analysis showed that OsSTLK overexpression remarkably reduced electrolyte leakage, malondialdehyde (MDA) content, reactive oxygen species (ROS) accumulation under salt stress conditions by up-regulating ROS-scavenging activities and modifying stomatal patterning. Moreover, Na+/K+ ratio and MAPK phosphorylation level were also reduced in OsSTLK-overexpression transgenic rice plants compared with WT control. Taken together, our findings suggested that OsSTLK as an important positive regulator of salt stress tolerance perhaps through regulating ROS scavenging system, Na+/K+ ratio and MAPK signal pathway.