Single-cell RNA-Seq profiling of human preimplantation embryos and embryonic stem cells
Single-cell RNA-Seq profiling of human preimplantation embryos and embryonic stem cells
复制标题
人类植入前胚胎和胚胎干细胞的单细胞 RNA 测序分析
DOI：
10.1038/nsmb.2660
复制
发表时间：
2013-09-01
影响因子：
16.8
通讯作者：
Tang, Fuchou
中科院分区：
文献类型：
--
作者：
Yan, Liying;Yang, Mingyu;Tang, Fuchou
测量单个细胞中的基因表达对于理解控制人类胚胎发育的基因调控网络至关重要。在这里，我们应用单细胞RNA测序(RNA-Seq)技术对124个来自人类植入前胚胎和不同代次的人类胚胎干细胞(HESCs)的单个细胞进行分析。在我们的数据集中检测到的母体表达基因数量为22,687个，其中包括8,701个长非编码RNA(LncRNAs)，这比之前通过cDNA微阵列检测到的9,735个母体基因有显著增加。我们发现了2733个新的lncRNA，其中许多在特定的发育阶段表达。为了解决人上皮细胞和体外培养的人胚胎干细胞的基因表达特征是否相同的问题，我们发现人上皮细胞和原代培养的人胚胎干细胞具有显著不同的转录产物，有1,498个基因在它们之间表现出差异表达。这项工作提供了人类早期胚胎和人类胚胎干细胞转录组景观的全面框架。
Measuring gene expression in individual cells is crucial for understanding the gene regulatory network controlling human embryonic development. Here we apply single-cell RNA sequencing (RNA-Seq) analysis to 124 individual cells from human preimplantation embryos and human embryonic stem cells (hESCs) at different passages. The number of maternally expressed genes detected in our data set is 22,687, including 8,701 long noncoding RNAs (lncRNAs), which represents a significant increase from 9,735 maternal genes detected previously by cDNA microarray. We discovered 2,733 novel lncRNAs, many of which are expressed in specific developmental stages. To address the long-standing question whether gene expression signatures of human epiblast (EPI) and in vitro hESCs are the same, we found that EPI cells and primary hESC outgrowth have dramatically different transcriptomes, with 1,498 genes showing differential expression between them. This work provides a comprehensive framework of the transcriptome landscapes of human early embryos and hESCs.