Human decidual cell biosynthesis of leukemia inhibitory factor: regulation by decidual cytokines and steroid hormones.
Human decidual cell biosynthesis of leukemia inhibitory factor: regulation by decidual cytokines and steroid hormones.
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人类蜕膜细胞白血病抑制因子的生物合成：蜕膜细胞因子和类固醇激素的调节。
DOI：
10.1095/biolreprod56.5.1274
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发表时间：
1997
影响因子：
3.6
通讯作者：
Y. Murata
中科院分区：
文献类型：
--
作者：
K. Sawai;N. Matsuzaki;T. Okada;K. Shimoya;M. Koyama;C. Azuma;F. Saji;Y. Murata
白血病抑制因子（LIF）的产生被认为是至关重要的成功植入到蜕膜的胚泡，因为LIF的表达是必不可少的小鼠胚细胞的植入。我们研究了蜕膜细胞因子和类固醇激素对LIF产生的调节作用。通过白细胞介素-1、肿瘤坏死因子α或转化生长因子β刺激蜕膜细胞以剂量依赖性方式增加LIF的产生。此外，雌二醇，一种在排卵和早孕期间增加的类固醇激素，也以剂量依赖性方式增强LIF的产生。这些反应被蛋白激酶C（PKC）抑制剂阻断，但不被其他激酶抑制剂，这表明PKC在细胞因子和雌二醇介导的蜕膜LIF产生中的重要作用。我们还发现，用LIF刺激蜕膜细胞不能刺激这些细胞中的DNA合成和催乳素产生。总之，LIF主要定位于蜕膜腺和基质，其生产增加的细胞因子和雌二醇的剂量依赖性的方式，但刺激蜕膜细胞的LIF并不影响其增殖或催乳素的生产。
The production of leukemia inhibitory factor (LIF) is suggested to be critical for the successful implantation of blastocysts into decidua, because LIF expression is essential for the implantation of mouse blastocytes. We investigated the regulation of LIF production by decidual cytokines and steroid hormones. Stimulation of decidual cells by interleukin-1, tumor necrosis factor alpha, or transforming growth factor beta augmented LIF production in a dose-dependent manner. Moreover, estradiol, a steroid hormone that increases during ovulation and early pregnancy, also enhanced LIF production in a dose-dependent manner. These responses were blocked by protein kinase C (PKC) inhibitor but not by other kinase inhibitors, suggesting an important role of PKC in decidual LIF production mediated by cytokines and estradiol. We also showed that stimulating decidual cells with LIF failed to stimulate DNA synthesis and prolactin production in these cells. In summary, LIF was mainly localized in the decidual glands and stroma, and its production was increased by cytokines and estradiol in a dose-dependent fashion; but stimulation of decidual cells by LIF did not influence their proliferation or their prolactin production.
使用聚合酶链式反应检测星形胶质细胞培养物中的细胞因子 mRNA。
DOI：
--
发表时间：
1990
期刊：
Lymphokine research
影响因子：
--
作者：
Wesselingh,SL;Gough,NM;Finlay-Jones,JJ;McDonald,PJ
通讯作者：
McDonald,PJ
影响因子：
--
作者：
S. F. Arnold;J. Obourn;H. Jaffe;A. Notides
通讯作者：
S. F. Arnold;J. Obourn;H. Jaffe;A. Notides