Response of articular chondrocytes to pituitary fibroblast growth factor (FGF).
Response of articular chondrocytes to pituitary fibroblast growth factor (FGF).
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关节软骨细胞对垂体成纤维细胞生长因子（FGF）的反应。
DOI：
10.1002/jcp.1041120109
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发表时间：
1982
影响因子：
5.6
通讯作者：
Malemud,CJ
中科院分区：
文献类型：
--
作者：
Sachs,BL;Goldberg,VM;Moskowitz,RW;Malemud,CJ
兔关节池软骨细胞在初代单层培养开始后附着在培养瓶上的时间被描绘出来，48小时后附着（48‐AT）或漂浮（48‐F）。我们还研究了一般的计时细胞群（72小时后附着，72‐AT）。研究了垂体成纤维细胞生长因子（FGF）的促生长活性及其对硫酸蛋白多糖合成的影响。FGF （100 ng/ml）刺激每个软骨细胞群在1小时脉冲中加入3H胸腺嘧啶。AT‐72软骨细胞对FGF的反应需要额外补充胎牛血清，而48‐F细胞的反应不依赖于血清。48‐AT软骨细胞对FGF （100 ng/ml）在3h‐胸腺嘧啶1小时脉冲作用下的反应在低血清（0.5-2.0%）条件下比在高血清（8-10%）条件下增强。在48‐AT和48‐F软骨细胞群体中，FGF减少了35so4与硫酸蛋白聚糖的结合，但在72‐AT群体中没有。48‐AT和48‐F软骨细胞中35so4掺入的减少并没有表现为通过Sepharose CL‐2B色谱法测量的硫酸化蛋白聚糖的水动力学大小的改变，也没有表现为产生的硫酸化糖胺聚糖类型的变化。这些结果表明，FGF对软骨细胞硫酸蛋白多糖合成产生了定量而非定性的改变。后者似乎与FGF对软骨细胞的促生长活性分离。
Rabbit chondrocytes from pooled articular joints have been delineated by their time of attachment of culture flasks after initiation of primary monolayer culture, either attached (48‐AT) or floating (48‐F) after 48 hours. A general population of chrondrocytes (attached after 72 hours, 72‐AT) was also studied. The growth‐promoting activity of pituitary fibroblast growth factor (FGF) and its effect on sulfated‐proteoglycan synthesis was studied on each chondrocyte population in secondary monolayer culture.3H‐thymidine incorporation during a 1‐hour pulse was stimulated by FGF (100 ng/ml) in each chondrocyte population. The response of AT‐72 chondrocytes to FGF required an additional fetal bovine serum supplement, while 48‐F cells resonded independent of serum. The response of 48‐AT chondrocytes to FGF (100 ng/ml) during a 1‐hour pulse with3H‐thymidine was increased in low serum (0.5–2.0%) rather than when high serum (8–10%) was present in the culture medium. FGF reduced35SO4incorporation into sulfated‐proteoglycans in the 48‐AT and 48‐F chondrocyte populations, but not in the 72‐AT population. The reduction in35SO4incorporation in the 48‐AT and 48‐F chondrocytes was not characterized by alterations in the hydrodynamic size of the sulfated‐proteoglycans as measured by Sepharose CL‐2B chromatography nor by changes in the types of sulfated‐glycosaminoglycans produced. These results indicated that FGF produced quantitative rather than qualitative alterations in chondrocyte sulfated‐proteoglycan synthesis. The latter appears uncoupled from the growth‐promoting activity of FGF on chondrocytes.