Radiation-inducible protein RbAp48 contributes to radiosensitivity of cervical cancer cells
Radiation-inducible protein RbAp48 contributes to radiosensitivity of cervical cancer cells
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辐射诱导蛋白 RbAp48 有助于宫颈癌细胞的放射敏感性
DOI：
10.1016/j.ygyno.2013.06.002
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发表时间：
2013-09-01
影响因子：
4.7
通讯作者：
Zhao, Weiming
中科院分区：
文献类型：
--
作者：
Zheng, Lin;Tang, Wei;Zhao, Weiming
目的。视网膜母细胞瘤相关蛋白48（RbAp48）近期被发现是一种放射敏感基因。我们旨在体内和体外研究RbAp48在宫颈癌细胞放射敏感性中的作用。
方法。我们采用实时逆转录聚合酶链反应（real - time RT - PCR）和蛋白质印迹法（Western blot assay）检测RbAp48在受辐射的宫颈癌细胞系（包括SiHa、Caslci和HeLa细胞）中的表达。通过细胞增殖、计数、存活和凋亡以及细胞周期和肿瘤生长实验（在RbAp48过表达或基因沉默的情况下）来评估RbAp48在宫颈癌细胞放射敏感性中的作用。
结果。RbAp48在受辐射的宫颈癌细胞系中的表达增加。RbAp48过表达诱导受辐射细胞发生G2/M期阻滞和凋亡，这与p53、Rb和半胱天冬酶 - 8（caspase - 8）表达上调有关。腺病毒 - RbAp48感染和辐射协同抑制裸鼠肿瘤生长。
结论。由于RbAp48在体内和体外均增强了宫颈癌细胞的放射敏感性，所以它是宫颈癌细胞中的一种辐射诱导基因。RbAp48可能是改善宫颈癌患者放射治疗效果的一个潜在靶点。（C）2013作者。由爱思唯尔公司出版。保留所有权利。
Objective. Retinoblastoma-associated protein 48 (RbAp48) has been recently discovered as a radiosensitive gene. We aimed to investigate the role of RbAp48 in radiosensitivity of cervical cancer cells in vivo and in vitro.Methods. We used real-time RT-PCR and Western blot assay to examine the expression of RbAp48 in irradiated cervical cancer cell lines, including SiHa, Caslci, and HeLa cells. The role of RbAp48 in radiosensitivity of cervical cancer cells was assessed by cell proliferation, counting, survival, and apoptosis as well as cell cycle and tumor growth assays with RbAp48 overexpression or gene silencing.Results. The expression of RbAp48 was increased in irradiated cervical cancer cell lines. Overexpression of RbAp48 induced G2/M arrest and apoptosis in irradiated cells, which was related to upregulation of p53, Rb and caspase-8 expression. Adenovirus-RbAp48 infection and irradiation synergistically inhibited tumor growth in nude mice.Conclusions. RbAp48 is a radiation-inducible gene in cervical cancer cells because of enhanced radiosensitivity of cervical cancer cells in vivo and in vitro. RbAp48 may be a potential target to improve the results of radiation therapy for patients with cervical cancer. (C) 2013 The Authors. Published by Elsevier Inc. All rights reserved.