Development and application of a comprehensive analytical workflow for the quantification of non-volatile low molecular weight lipids on archaeological stone tools
Development and application of a comprehensive analytical workflow for the quantification of non-volatile low molecular weight lipids on archaeological stone tools
复制标题
考古石器上非挥发性低分子量脂质定量综合分析工作流程的开发和应用
DOI：
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
R. Roberts
中科院分区：
文献类型：
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作者：
Susan Luong;E. Hayes;Emma N. Flannery;T. Sutikna;M. Tocheri;E. W. Saptomo;Jatmiko;R. Roberts
由于与保存、污染和多种来源的残留物有关的问题，史前石器上使用相关残留物的来源确定尤其具有挑战性。为了增加对这一过程的信心，开发了一个分析工作流程，包括：(1)保留回收残留物空间信息的采样策略，并能够监测环境污染；(2)采用灵敏、选择性的气相色谱-串联质谱（GC-MS/MS）方法定量石制文物上的非挥发性低分子量脂质。此工作流应用于14石头从存款Liang Bua文物发掘,印尼弗洛勒斯岛的石灰岩洞。这些人工制品的年龄范围在~ 14000年到1000年之间，使用低倍显微镜初步分类为可能显示使用痕迹（n = 7）和不显示使用痕迹（n = 7）。残留物通过直接溶剂萃取法从人工制品表面取样。等价物被加入内部标准并衍生。利用优化的多重反应监测（MRM）转换对40种饱和脂肪酸、甾醇、二萜类和三萜类及其类似物的三甲基硅基衍生物进行了定量分析。六件可能使用的人工制品含有甾醇、植物甾醇和萜类化合物，无论是单独的还是组合的，而这些化合物在七件初步归类为未使用的人工制品中都没有普遍发现。这表明这六个工件可能被用作处理资源的工具，并为进一步的研究提供了空间。这个工作流程也可以适用于其他考古对象的分析。
Source determination of use-related residues on prehistoric stone tools is especially challenging, due to issues related to preservation, contamination and the contribution of residues from multiple sources. To increase confidence in this process, an analytical workflow was developed to include: (1) a sampling strategy that retains spatial information of the recovered residues and enables monitoring of environmental contamination; and (2) a sensitive and selective gas chromatography-tandem mass spectrometry (GC-MS/MS) procedure to quantify non-volatile low molecular weight lipids on stone artefacts. This workflow was applied to 14 stone artefacts excavated from deposits at Liang Bua, a limestone cave on the Indonesian island of Flores. These artefacts range in age between ∼14 000 and 1000 years old, and were preliminarily classified as either potentially showing traces of use (n = 7) or not (n = 7) using low magnification microscopy. Residues were sampled by direct solvent extraction off the surface of the artefacts. The aliquots were spiked with internal standards and derivatised. The trimethylsilyl derivatives of 40 saturated fatty acids, sterols, di- and tri-terpenoids and their analogues were quantified using optimised multiple reaction monitoring (MRM) transitions. Six of the potentially used artefacts contained sterols, phytosterols and terpenoids, either individually or in combination, whereas none of these compounds was commonly found on the seven artefacts preliminarily classified as unused. This suggests that these six artefacts may have been used as implements to process resources, and provides scope for further investigation. This workflow can also be adapted for the analysis of other archaeological objects.
气相色谱质谱法检测考古陶器有机残留物“结合”相中保存的二羟基脂肪酸。
DOI：
10.1002/rcm.5038
发表时间：
2011
期刊：
RCM
影响因子：
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作者：
Hansel FA
通讯作者：
Hansel FA