First fungemia case due to environmental yeast Wickerhamomyces myanmarensis: detection by multiplex qPCR and antifungal susceptibility
First fungemia case due to environmental yeast Wickerhamomyces myanmarensis: detection by multiplex qPCR and antifungal susceptibility
复制标题
首例由环境酵母缅甸威克哈姆酵母引起的真菌血症病例：通过多重 qPCR 和抗真菌药敏检测
DOI：
10.2217/fmb-2018-0253
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发表时间：
2019-03-01
影响因子：
3.1
通讯作者：
Boekhout, Teun
中科院分区：
文献类型：
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作者：
Arastehfar, Amir;Bakhtiari, Mina;Boekhout, Teun
目的：报道缅甸Wickerhamomyces myanmarensis的临床首例。患者与方法：从一名5.5岁男性受试者的血液和中心静脉导管中分离酵母细胞。使用API 20 C AUX、MALDI-TOF MS、ITS和LSU rDNA测序以及我们的qPCR测定法进行鉴定，并通过CLSI M27-A3测定MIC值。结果：ITS和LSU rDNA序列分析表明，两株菌均为W.而API 20 C AUX和MALDI-TOF MS不能正确鉴定它们。我们的qPCR特别区分了W. myanmarensis），从W.阿古斯血液分离株对氟康唑、伏立康唑和泊沙康唑的MIC值较高。结论：利用可靠的鉴定工具可以揭示机会酵母菌的真正谱。
Aim: Presenting the first clinical case of Wickerhamomyces myanmarensis. Patients & methods: Yeast cells were isolated from blood and central venous catheter of a 5.5-year-old male subject. API 20C AUX, MALDI-TOF MS, ITS and LSU rDNA sequencing, and our qPCR assay were used for identification and the MIC values were determined by CLSI M27-A3. Results: ITS and LSU rDNA sequencing identified both isolates as W. myanmarensis, while API 20C AUX and MALDI-TOF MS did not identify them correctly. Our qPCR specifically distinguished W. myanmarensis from W. anomalus. Isolate obtained from blood showed a higher MIC value for fluconazole, voriconazole and posaconazole. Conclusion: Utilization of reliable identification tools might reveal the genuine spectrum of opportunistic yeast species.