Monitoring the permeability of the nuclear envelope during the cell cycle
Monitoring the permeability of the nuclear envelope during the cell cycle
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DOI：
10.1016/j.ymeth.2005.07.010
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发表时间：
2006-01-01
期刊：
影响因子：
4.8
通讯作者：
Ellenberg, J
中科院分区：
文献类型：
--
作者：
Lénárt, P;Ellenberg, J
In animal organisms the nuclear envelope (NE) disassembles during cell division resulting in complete intermixing of cytoplasmic and nuclear compartments. This leads to the activation of many mitotic enzymes, which were kept away from their substrates or regulators by nuclear or cytoplasmic sequestration in interphase. Nuclear envelope breakdown (NEBD) is thus an essential step of mitotic entry and commits a cell to M-phase. NEBD begins with the partial disassembly of nuclear pore complexes, leading to a limited permeabilization of the NE for molecules up to similar to 40 nm diameter. This is followed by the complete disruption of nuclear pores, which causes local fenestration of the double nuclear membrane and subsequently breakdown of the entire NE structure. Here, we describe the use of different sized inert fluorescent tracer molecules to directly visualize these different steps of NEBD in live cells by fluorescence microscopy. (c) 2005 Elsevier Inc. All rights reserved.
