Development of strain-specific PCR reactions for the detection of the probiotic Escherichia coli strain Nissle 1917 in fecal samples
Development of strain-specific PCR reactions for the detection of the probiotic Escherichia coli strain Nissle 1917 in fecal samples
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DOI：
10.1016/s0923-2508(02)00007-4
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发表时间：
2003-01-01
影响因子：
2.6
通讯作者：
Hacker, J
中科院分区：
文献类型：
--
作者：
Blum-Oehler, G;Oswald, S;Hacker, J
PCR 用于建立非致病性大肠杆菌菌株 Nissle 1917 (DSM6601) 的特异性检测系统，该菌株被用作对抗肠道紊乱和疾病的益生菌药物。已开发出五种 PCR 检测方法，这些检测方法基于染色体编码的主要菌毛亚基基因 fimA（1 型菌毛）和 focA（F1C 菌毛）以及两个小型神秘质粒 pMUT1 和 pMUT2。通过测试来自不同来源的 354 种不同的致病性和非致病性大肠杆菌菌株，包括大肠杆菌 K-12、粪便和环境以及致病性肠外和肠道大肠杆菌菌株，对这些测定进行了验证。最具体的结果是使用基于质粒 pMUT2 的 DNA 序列的引物获得的。所描述的基于质粒的 PCR 测定可用于检测患者粪便中的大肠杆菌菌株 Nissle 1917，无需事先培养。 (C) 2002 年科学与医学版 Elsevier SAS。版权所有。
PCR was used to establish a specific detection system for the non-pathogenic Escherichia coli strain Nissle 1917 (DSM6601), which is used as a probiotic drug against intestinal disorders and diseases. Five PCR assays have been developed which are based on the chromosomally encoded major fimbrial subunit genes fimA (type 1 fimbriae) and focA (F1C fimbriae), and the two small cryptic plasmids pMUT1 and pMUT2. The assays were validated by testing a collection of 354 different pathogenic and non-pathogenic E. coli strains from various origins, including E. coli K-12, fecal and environmental as well as pathogenic extraintestinal and intestinal E. coli strains. The most specific results were obtained with primers based on DNA sequences from plasmid pMUT2. The plasmid-based PCR assays described can be used to detect E. coli strain Nissle 1917 in feces from patients without prior cultivation. (C) 2002 Editions scientifiques et medicales Elsevier SAS. All rights reserved.