The Lipopolysaccharide from Pseudomonas aeruginosa NCTC 8505
The Lipopolysaccharide from Pseudomonas aeruginosa NCTC 8505
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铜绿假单胞菌脂多糖 NCTC 8505
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
S. Wilkinson
中科院分区：
文献类型：
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作者：
Y. Tahara;S. Wilkinson
对HABS 03型铜绿假单胞菌NCTC8505脂多糖的O-特异组分进行了结构研究。O-特异性多糖含有一个四糖重复单元，由L鼠李糖(Rha)、2-乙氨基-2-脱氧-d-葡萄糖(GlcNAc)、2-乙酰胺-2-脱氧-L-半乳糖醛酸(GalNAcA)和2，4-二乙酰胺-2，4，6-三脱氧-d-葡萄糖(BacNAc2)残基组成。已将以下结构分配给重复单元：
3)Rhap(β16)GlcpNAc(α14)GalpNAcA(α13)BacpNAc2(α1.
亲本脂多糖是S、R和SR种的混合物，其高磷含量部分是由于存在三磷酸残基，与其他来自铜绿假单胞菌的脂多糖一样。除了磷、七糖、3-脱氧辛磺酸和酰胺结合的丙氨酸外，核心低聚糖还含有葡萄糖、鼠李糖和氨基半乳糖(摩尔比为3：1：1)。鼠李糖和部分葡萄糖以未取代的吡喃糖苷残基存在：其他葡萄糖残基是6-取代的。
Structural studies have been carried out on the O-specific fraction from the lipopolysaccharide of Pseudomonas aeruginosa NCTC 8505, Habs serotype 03. The O-specific polysaccharide has a tetrasaccharide repeating-unit containing residues of l-rhamnose (Rha), 2-acetamido-2-deoxy-d-glucose (GlcNAc), 2-acetamido-2-deoxy-l-galacturonic acid (GalNAcA), and 2,4-diacetamido-2,4,6-trideoxy-d-glucose (BacNAc2). The following structure has been assigned to the repeating-unit:
3)Rhap(β16)GlcpNAc(α14) GalpNAcA(α13)BacpNAc2(α1.
The parent lipopolysaccharide is a mixture of S, R, and SR species, and its high phosphorus content is partly due to the presence of triphosphate residues, as found for other lipopolysaccharides from P. aeruginosa. In addition to phosphorus, heptose, a 3-deoxyoctulosonic acid, and amide-bound alanine, the core oligosaccharide contains glucose, rhamnose, and galactosamine (molar proportions 3:1:1). The rhamnose and part of the glucose are present as unsubstituted pyranoside residues: other glucose residues are 6-substituted.