Electrophysiological effects of sustained delivery of CRF and its receptor agonists in hippocampal slices
Electrophysiological effects of sustained delivery of CRF and its receptor agonists in hippocampal slices
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DOI：
10.1016/s0006-8993(01)03160-2
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发表时间：
2001-12-13
期刊：
影响因子：
2.9
通讯作者：
Izvarina, N
中科院分区：
文献类型：
--
作者：
Rebaudo, R;Melani, R;Izvarina, N
促肾上腺皮质激素释放因子 (CRF) 是一种下丘脑肽，可调节促肾上腺皮质激素 (ATCH) 和 β-内啡肽的释放。有人认为它可以调节大鼠的学习和记忆过程。然而，迄今为止，CRF 对海马神经元产生的电生理效应的研究还很少。特别是，CRF 对长时程增强 (LTP) 的影响尚不清楚，这种现象被认为是记忆过程的基础。我们研究了持续施用 CRF 及其两种受体激动剂对基础神经元活性和体外海马 LTR 的影响。这两种受体激动剂是 D-Glu-20-CRF 和 D-Pro-5-CRF，分别对 CRF-R I 和 CRF-R2 受体具有选择性。我们发现浓度为 1 nM 的 CRF、D-Pro-5-CRF 和 D-Glu-20-CRF 会降低海马群体峰值的幅度并防止 LTR 的发生。较高浓度的 CFR 对神经元活动的抑制作用较小，但它们仍然可以防止 LTR 的发生 (C) 2001 Elsevier Science B.V. 保留所有权利。
The corticotropin-releasing factor (CRF) is a hypothalamic peptide that regulates the release of adrenocorticotropic hormone (ATCH) and of beta -endorphin. It has been suggested that it modulates learning and memory processes in rat. However, the electrophysiological effects that CRF produces on hippocampal neurons have been so far little investigated. In particular, the effects of CRF on long-term potentiation (LTP), a phenomenon which is thought to be the substrate of memory processes, are unknown. We studied the effects of sustained administration of CRF and of two of its receptor agonists on basal neuronal activity and on in vitro hippocampal LTR The two receptor agonists were D-Glu-20-CRF and D-Pro-5-CRF, selective for the CRF-R I and the CRF-R2 receptors, respectively. We found that CRF, D-Pro-5-CRF and D-Glu-20-CRF at the concentration of 1 nM diminish the amplitude of hippocampal population spike and prevent the onset of LTR Higher concentrations of CFR have less depressing effects on neuronal activity, yet they still prevent the occurrence of LTR (C) 2001 Elsevier Science B.V. All rights reserved.