Cultured rabbit gastric epithelial cells producing prostaglandin I2.
Cultured rabbit gastric epithelial cells producing prostaglandin I2.
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培养的兔胃上皮细胞产生前列腺素I2。
DOI：
10.1016/0016-5085(83)90073-2
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发表时间：
1983
期刊：
影响因子：
29.4
通讯作者：
Sei
中科院分区：
文献类型：
--
作者：
Koozi Matuoka;Masanobu Tanaka;Youji Mitsui;Sei
本文报道了一种用中性蛋白酶Dispase纯化兔胎胃粘膜细胞的新方法。这些细胞形成一个典型的多边形单层后，去除成纤维细胞样细胞的分散酶处理，并保留了“正常”细胞的功能，表现出体外老化与有限的寿命。培养物主要由表面粘液细胞（占总细胞群的50%-60%）组成，并产生异甘草素（主要是I2）。在培养基中加入乙酰水杨酸可显著抑制培养的胃上皮细胞产生前列腺素。该细胞株可用于胃细胞保护作用的研究。
A new method with Dispase, a bacterial neutral protease, was developed for purification of gastric mucosal cells from rabbit fetuses. These cells formed a typical polygonal monolayer after removal of fibroblastlike cells by Dispase treatment, and preserved the features of “normal” cells, exhibiting in vitro aging with a finite life span. The culture consisted mainly of surface mucous cells (50%–60% of the total cell population) and produced prostaglandins (predominantly I2). The addition of acetyl-salicylic acid to the culture medium caused marked inhibition of prostaglandin production by the cultured gastric epithelial cells. This epithelial cell strain will be useful in studies on cytoprotection of the stomach.