Computerized analysis of audible and ultrasonic vocalizations of rats as a standardized measure of pain-related behavior
Computerized analysis of audible and ultrasonic vocalizations of rats as a standardized measure of pain-related behavior
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DOI：
10.1016/j.jneumeth.2004.07.005
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发表时间：
2005-02-15
影响因子：
3
通讯作者：
Neugebauer, V
中科院分区：
文献类型：
--
作者：
Han, JS;Bird, GC;Neugebauer, V
实验疼痛的行为评估是分析疼痛机制和验证治疗靶点的必要条件。尤其是关节炎的疼痛，与消极的情感状态和失调有显著的关系。在这里，我们提出了一种标准化的方法，用于定量分析清醒大鼠的声音和超声波（25 +/- 4 kHz）发声，作为关节炎疼痛模型中更高综合行为的衡量标准。用蝙蝠检测器和电容式麦克风分别记录超声和可听的声音，以响应无害和有害的机械刺激的膝盖在诱导急性关节炎之前和之后的一个膝盖。计算机系统用于分析滤波信号的数量和持续时间。对于行为测试，动物被放置在一个定制的记录室中，以确保一致的刺激应用和稳定的记录，并消除任何运动引起的噪音。有害刺激比无害刺激产生更强的发声。在急性关节炎诱导后，对无害（异常性疼痛）和有害（痛觉过敏）刺激的听觉和超声发声均增加。在相同的动物中，这些变化伴随着膝关节周长增加，后肢退缩阈值降低和探索行为减少。听觉和超声发声的计算机分析是一种有效、定量、可靠和方便的测量疼痛相关行为的方法。(C) 2004 Elsevier B.V.版权所有
The behavioral assessment of experimental pain is essential for the analysis of pain mechanisms and the validation of therapeutic targets. Arthritic pain, in particular, is significantly associated with negative affective states and disorders. Here we present a standardized method for the quantitative analysis of audible and ultrasonic (25 +/- 4 kHz) vocalizations in awake rats as a measure of higher integrated behavior in a model of arthritic pain. A bat detector and a condenser microphone were used to record ultrasonic and audible vocalizations, respectively, in response to innocuous and noxious mechanical stimulation of the knee before and after induction of acute arthritis in one knee. A computerized system was used to analyze number and duration of the filtered signals. For the behavioral tests, the animal was placed in a customized recording chamber to ensure consistent stimulus application and stable recordings and to eliminate any movement-induced noise. Noxious stimuli produced stronger vocalizations than innocuous stimuli. Both audible and ultrasonic vocalizations to innocuous (allodynia) and noxious (hyperalgesia) stimuli increased after the induction of acute arthritis. These changes were accompanied by increased knee joint circumference, lowered hind limb withdrawal thresholds and reduced exploratory behavior in the same animals. The computerized analysis of audible and ultrasonic vocalizations is a valid, quantitative, reliable and convenient method to measure pain-related behavior.(C) 2004 Elsevier B.V. All rights reserved.