Bifunctional anticaries peptides with antibacterial and remineralizing effects
Bifunctional anticaries peptides with antibacterial and remineralizing effects
复制标题
具有抗菌和再矿化作用的双功能防龋肽
DOI：
10.1111/odi.12990
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发表时间：
2018
期刊：
影响因子：
3.8
通讯作者：
wang xiuqing
中科院分区：
文献类型：
--
作者：
zhang linglin;wang xiuqing
目的初步龋病是临床上常见的龋病。减少致龋菌和促进再矿化是防治龋病的有效途径。本研究旨在开发具有抗菌和再矿化特性的双功能抗肽。方法设计多肽TDH19、TNH19和TVH19，并通过比较其对变形链球菌的最低抑菌浓度（mic）和最低杀菌浓度（MBCs）及其矿化作用，筛选出一种多肽。然后通过(a)效应对所选肽的双功能进行研究。(b)对初始病变的再矿化作用和(c)唾液的稳定性和对人口腔角质形成细胞（HOKs）的细胞相容性。结果stvh19的MIC和MBC最低，矿化能力较好。抑制了新生物膜的形成，降低了旧生物膜的活力（p< 0.05）。用TVH19治疗初发龋，表面显微硬度恢复更快，损伤深度更浅，矿物质含量更高（p< 0.05）。TVH19与NaF样品间差异无统计学意义（p < 0.05）。TVH19在唾液中稳定，对HOKs的影响较小。结论新型双功能抗龋肽TVH19具有显著的抗菌活性和促进初发龋再矿化的潜力。
ObjectivesInitial dental caries often occurs in clinic. Reduction of cariogenic bacteria and promotion of remineralization are effective ways to control them. This study was to develop bifunctional anticaries peptides with antibacterial and remineralizing properties.MethodsWe designed peptides TDH19, TNH19, and TVH19 and selected one through comparing their minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) againstStreptococcus mutansand their reaction on mineralization. Then the bifunction of the selected peptide was studied through: (a) effects onS. mutansbiofilm, (b) remineralizing effects on initial lesions and (c) stability in saliva and cytocompatibility to human oral keratinocytes (HOKs).ResultsTVH19 showed the lowest MIC and MBC and a better mineralizing ability. It inhibited new biofilm formation and reduced the viability of old biofilm (p< 0.05). Treating initial caries with TVH19 led to greater recovery of surface microhardness, shallower lesion depth, and higher mineral content (p< 0.05). No significant differences were observed between TVH19 and NaF samples (p> 0.05). TVH19 was stable in saliva and had little effect on HOKs.ConclusionsThe novel bifunctional anticaries peptide TVH19 was developed with remarkable antibacterial activity and the potential to enhance remineralization of initial caries.