A novel strategy for succinate and polyhydroxybutyrate co-production in Escherichia coli
A novel strategy for succinate and polyhydroxybutyrate co-production in Escherichia coli
复制标题
大肠杆菌联产琥珀酸和聚羟基丁酸的新策略
DOI：
10.1016/j.biortech.2010.04.084
复制
发表时间：
2010-10-01
影响因子：
11.4
通讯作者：
Qi, Qingsheng
中科院分区：
文献类型：
--
作者：
Kang, Zhen;Gao, Cuijuan;Qi, Qingsheng
基于代谢分析，在大肠杆菌 MG1655 中设计并改造了琥珀酸和聚羟基丁酸（PHB）共生产途径。工程化大肠杆菌的批量培养表明，它能够同时积累细胞外琥珀酸和细胞内 PHB。 PHB的积累不仅提高了琥珀酸的产量，而且减少了丙酮酸和乙酸的分泌。消耗45 g l(-1) 葡萄糖后，大肠杆菌QZ1112 在分批发酵中积累了24.6 g l(-1) 琥珀酸和4.95 g l(-1) PHB。 PHB 含量达到其细胞干重的 41.3 wt.%，这表明细胞碎片可以用作增值副产品。 (C) 2010 Elsevier Ltd. 保留所有权利。
Based on the metabolic analysis, a succinate and polyhydroxybutyrate (PHB) co-production pathway was designed and engineered in Escherichia coli MG1655. Batch cultivation of the engineered E. coli revealed that it was able to accumulate both extracellular succinate and intracellular PHB simultaneously. PHB accumulation not only improved succinate production, but also reduced pyruvate and acetate secretion. With a consumption of 45 g l(-1) glucose, E. coli QZ1112 was shown to accumulate 24.6 g l(-1) succinate and 4.95 g l(-1) PHB in batch fermentation. The PHB content reached 41.3 wt.% of its cell dry weight, which suggested that the cell debris can be used as value added by-product. (C) 2010 Elsevier Ltd. All rights reserved.