The Use of Campenot Trichambers for the Study of Peripheral Neuronal Growth and Survival in Presence of Thrombotic Factors and Serpins.
The Use of Campenot Trichambers for the Study of Peripheral Neuronal Growth and Survival in Presence of Thrombotic Factors and Serpins.
复制标题
使用 Campenot Trichambers 研究血栓因子和丝氨酸蛋白酶抑制剂存在下的周围神经元生长和存活。
DOI：
10.1007/978-1-0716-2835-5_8
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Hogue,IanB
中科院分区：
文献类型：
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作者：
Tierney,WesleyM;Hogue,IanB
神经系统损伤，如脊髓损伤(SCI)、创伤性脑损伤(TBI)和周围神经损伤等，其发生机制复杂，目前尚不清楚。在急性组织损伤和细胞死亡之后，炎症过程会造成持续的损害。许多因素调节这种炎症，包括调节趋化因子表达的因素。丝氨酸蛋白酶，包括血栓和溶栓途径中的那些(如凝血酶、tPA、uPA)在神经系统损伤时上调，可以调节许多趋化因子的释放和生物利用度。病毒衍生的免疫调节剂，如丝氨酸蛋白酶抑制剂(SERPIN)SERP-1，通过减少炎症和组织损伤而起到保护作用。然而，SERP-1神经保护的确切机制仍在研究中。体外分离的神经元培养系统，如Campenot三室，对这种机制研究是有用的。本章提供了在Campenot三轮室中组装和培养啮齿动物胚胎颈上神经节(SCG)和背根神经节(DRG)神经元的方案，以及通过这些方法实现的实验类型的指导性例子。
The mechanisms underlying nervous system injury, such as spinal cord injury (SCI), traumatic brain injury (TBI), and peripheral nerve injury are complex and not well understood. Following acute tissue damage and cell death, inflammatory processes cause ongoing damage. Many factors regulate this inflammation, including factors that modulate chemokine expression. Serine proteases, including those of the thrombotic and thrombolytic pathways (e.g., thrombin, tPA, uPA) are upregulated during nervous system damage and can modulate the release and bioavailability of many chemokines. Virus-derived immunomodulators, such as Serp-1, a serine protease inhibitor (serpin), have protective effects by reducing inflammation and tissue damage. However, the precise mechanisms of Serp-1 neuroprotection are still being studied. Compartmentalized in vitro neuron culture systems, such as the Campenot trichamber, are useful for such mechanistic studies. This chapter provides a protocol for assembling and culturing rodent embryonic superior cervical ganglion (SCG) and dorsal root ganglion (DRG) neurons in Campenot trichambers, as well as instructive examples of the types of experiments enabled by these methods.