THE ACTION OF CALCITONIN GENE-RELATED PEPTIDE ON ASTROCYTE MORPHOLOGY AND CYCLIC-AMP ACCUMULATION IN ASTROCYTE CULTURES FROM NEONATAL RAT-BRAIN
THE ACTION OF CALCITONIN GENE-RELATED PEPTIDE ON ASTROCYTE MORPHOLOGY AND CYCLIC-AMP ACCUMULATION IN ASTROCYTE CULTURES FROM NEONATAL RAT-BRAIN
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DOI：
10.1016/0304-3940(91)90237-n
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发表时间：
1991-09-02
影响因子：
2.5
通讯作者：
KREUTZBERG, GW
中科院分区：
文献类型：
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作者：
LAZAR, P;REDDINGTON, M;KREUTZBERG, GW
使用从 1 至 2 日龄大鼠大脑中获得的 9 至 14 日龄星形胶质细胞培养物研究了降钙素基因相关肽 (CGRP) 的作用。通过神经胶质原纤维酸性蛋白（GFAP）的免疫荧光染色监测细胞的形态学外观，并使用放射免疫测定法测量培养物中腺苷3',5'-单磷酸（环AMP）的浓度。 CGRP 诱导大鼠大脑培养的星形胶质细胞形态发生变化。 GFAP 阳性的扁平、多边形细胞变得多极，具有许多过程。此外，CGRP 刺激环 AMP 的积累高达 30 倍，在 CGRP 浓度范围为 100-300 nM 时获得最大刺激的一半。这些数据提供了星形胶质细胞上存在功能性 CGRP 受体的证据，并表明 CGRP 作为控制星形胶质细胞分化和激活状态的细胞间信使的作用。
The actions of calcitonin gene-related peptide (CGRP) were investigated on 9- to 14-day-old astrocyte cultures obtained from the cerebra of 1- to 2-day-old rats. The morphological appearance of the cells was monitored by immunofluorescent staining of glial fibrillary acidic protein (GFAP), and the concentration of adenosine 3',5'-monophosphate (cyclic AMP) was measured in the cultures using radioimmunoassay. CGRP induced a morphological change in cultured astrocytes from rat brain. Flat, polygonal cells positive for GFAP became multipolar with many processes. In addition, CGRP stimulated the accumulation of cyclic AMP by up to 30-fold, half maximal stimulation being obtained at CGRP concentrations in the range 100-300 nM. These data provide evidence for the presence of functional CGRP receptors on astrocytes and suggest a role for CGRP as an intercellular messenger controlling the state of differentiation and activation of astroglial cells.