Interacting climate change factors (CO2 and temperature cycles) effects on growth, secondary metabolite gene expression and phenotypic ochratoxin A production by Aspergillus carbonarius strains on a grape-based matrix
Interacting climate change factors (CO2 and temperature cycles) effects on growth, secondary metabolite gene expression and phenotypic ochratoxin A production by Aspergillus carbonarius strains on a grape-based matrix
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DOI：
10.1016/j.funbio.2019.11.001
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发表时间：
2021-01-28
期刊：
影响因子：
2.5
通讯作者：
Perrone, Giancarlo
中科院分区：
文献类型：
--
作者：
Cervini, Carla;Verheecke-Vaessen, Carol;Perrone, Giancarlo
关于气候变化（CC）可能对葡萄的碳曲霉和赭曲霉毒素A（OTA）污染的影响知之甚少，特别是在地中海地区，在CC情景中，温度预计将增加+2-5摄氏度，二氧化碳从400增加到800/1200 ppm。本研究考察了（i）在交替的11.5 h黑暗/12.5 h光照周期中电流和升高的温度的影响（15-28摄氏度对18-34摄氏度），代表北普利亚地区，南意大利和（ii）现有和预测的二氧化碳浓度（400 vs 1000 ppm），对生长、生物合成基因表达的影响（AcOTApks、AcOTAnrps、AcOTAhal、AcOTAp 450、AcOTAbZIP）和天鹅绒复合物的调节基因（laeA/veA/velB，“velvet complex”）参与了3株A.卡本留斯在葡萄基质上进行的实验表明，CO2浓度升高导致生长和OTA产生的一般刺激。这些结果也得到了参与OTA生物合成的结构和调控基因的上调的支持。我们的研究首次表明，地中海地区二氧化碳浓度的升高可能会导致葡萄酒生产链中OTA污染的风险增加。(C)2019年英国真菌学会由爱思唯尔有限公司出版。保留所有权利。
Little is known on the impact that climate change (CC) may have on Aspergillus carbonarius and Ochratoxin A (OTA) contamination of grapes, especially in the Mediterranean region where in CC scenarios temperature are expected to increase by +2-5 degrees C and CO2 from 400 to 800/1200 ppm. This study examined the effect of (i) current and increased temperature in the alternating 11.5 h dark/12.5 h light cycle (15-28 degrees C vs 18-34 degrees C), representative of the North Apulia area, South Italy and (ii) existing and predicted CO2 concentrations (400 vs 1000 ppm), on growth, expression of biosynthetic genes (AcOTApks, AcOTAnrps, AcOTAhal, AcOTAp450, AcOTAbZIP) and regulatory genes of Velvet complex (laeA/veA/velB, "velvet complex") involved in OTA biosynthesis and OTA phenotypic production by three strains of A. carbonarius. The experiments made on a grape-based matrix showed that elevated CO2 resulted in a general stimulation of growth and OTA production. These results were also supported by the up regulation of both structural and regulatory genes involved in the OTA biosynthesis. Our work has shown for the first time that elevated CO2 concentration in the Mediterranean region may result in an increased risk of OTA contamination in the wine production chain. (C) 2019 British Mycological Society. Published by Elsevier Ltd. All rights reserved.