TGF-BETA EXPRESSION IN THE HUMAN COLON - DIFFERENTIAL IMMUNOSTAINING ALONG CRYPT EPITHELIUM
TGF-BETA EXPRESSION IN THE HUMAN COLON - DIFFERENTIAL IMMUNOSTAINING ALONG CRYPT EPITHELIUM
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DOI：
10.1038/bjc.1993.301
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发表时间：
1993-07-01
影响因子：
8.8
通讯作者：
MOORGHEN, M
中科院分区：
文献类型：
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作者：
AVERY, A;PARASKEVA, C;MOORGHEN, M
采用免疫组织化学方法检测大肠癌、腺瘤和正常大肠粘膜组织中TGF-β的表达。TGF-β的免疫反应性存在于52的58个正常粘膜检查的样品。在腺瘤和癌中，分别在10个样本中的8个和48个样本中的46个中观察到TGF-β表达，并且主要限于上皮细胞。在正常粘膜中，上皮细胞内存在TGF-β的差异表达，与增殖区室中的细胞相比，隐窝上部的上皮细胞显示出增强的免疫反应性。这种差异染色模式与TGF-β在控制结肠粘膜生长和分化中具有重要作用一致。
Samples of colorectal carcinoma, adenoma and normal colorectal mucosa were examined for the expression of TGF-beta by immunohistochemistry. Immunoreactivity for TGF-beta was present in 52 out of a total of 58 samples of normal mucosa examined. In adenomas and carcinomas TGF-beta expression was observed in eight out of ten and 46 out of 48 samples respectively and was largely restricted to epithelial cells. In normal mucosa differential expression of TGF-beta was present within epithelial cells, those in the upper parts of the crypts showing enhanced immunoreactivity compared to cells in the proliferative compartment. This pattern of differential staining is consistent with TGF-beta having an important role in the control of growth and differentiation in colonic mucosa.