Identification of a 50,000 Mr protein from rabbit brush border membranes that binds Clostridium perfringens enterotoxin.
Identification of a 50,000 Mr protein from rabbit brush border membranes that binds Clostridium perfringens enterotoxin.
复制标题
鉴定来自兔刷状缘膜的 50,000 Mr 蛋白，该蛋白与产气荚膜梭菌肠毒素结合。
DOI：
10.1016/0006-291x(83)91731-x
复制
发表时间：
1983
影响因子：
3.1
通讯作者：
McClane,BA
中科院分区：
文献类型：
--
作者：
Wnek,AP;McClane,BA
用NP-40从兔小肠刷状缘膜中提取结合产气荚膜梭菌肠毒素的蛋白。该蛋白通过肠毒素偶联溴化氰活化的Sepharose 4 B亲和层析进行部分纯化。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定，该蛋白质的分子量约为50，000。含有该蛋白的亲和纯化样品特异性抑制Vero（非洲绿色猴肾）细胞上肠毒素的生物活性。这些研究表明，这种蛋白质可能参与了肠毒素与兔肠上皮细胞的结合。
A protein that binds Clostridium perfringens enterotoxin was extracted with NP-40 from rabbit intestinal brush border membranes. This protein was partially purified by affinity chromatography on enterotoxin-coupled CNBr-activated Sepharose 4B. The molecular weight of this protein was approximately 50,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Affinity-purified samples containing this protein specifically inhibited biological activity of the enterotoxin on Vero (African green monkey kidney) cells. These studies suggest that this protein may be involved in the binding of the enterotoxin to rabbit intestinal epithelial cells.