A VNTR isolated by size selection of human DNA fragments detects RFLPs at the extremity of 1p and 4q.
A VNTR isolated by size selection of human DNA fragments detects RFLPs at the extremity of 1p and 4q.
复制标题
通过人类 DNA 片段大小选择分离的 VNTR 可检测 1p 和 4q 末端的 RFLP。
DOI：
10.1093/nar/19.16.4572
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发表时间：
1991
影响因子：
14.9
通讯作者：
G. Vergnaud
中科院分区：
文献类型：
--
作者：
D. Mariat;V. Lauthier;G. Vergnaud
来源/描述：Sau3A 总消化后 1.5 kb 以上的人类基因组 DNA 片段用于构建文库（使用 Puc 18 载体和 TG1 菌株）。分离、培养 50 个克隆，并用 HaeHL 和 HinfI 消化质粒微量制剂 (1)。最大的 DNA 插入片段（称为 CEB8）在用两种酶消化后大小为 3 kb。它被用作 CEPH 家族组上的探针 (2)。在高严格性下，CEB8 检测到两个基因座，可以根据条带强度进行区分（图 1）（在较低严格性下观察到许多多态性条带）。多态性：HaeIH 消化中使用的 CEB8 识别出两个多态性位点，可通过它们的相对强度进行区分（图 1）。
Source/Description: Human genomic DNA fragments above 1.5 kb after an Sau3A total digestion were used to construct a library (using a Puc 18 vector and a TG1 bacterial strain). Fifty clones were isolated, grown and plasmidminipreparations (1) were digested with HaeHL and HinfI. The largest DNA insert called CEB8 was 3 kb in size after digestion with both enyzmes. It was used as a probe (2) on the panel of CEPH families.At a high stringency CEB8 detects two loci which can be discriminated on the basis of band intensities (Fig. 1)(at a lower stringency many polymorphic bands are observed). Polymorphism: CEB8 usedon HaeIH digestions identifies two polymorphic loci, distinguishable by their relative intensities (Fig. 1).