Myosin cross-bridge orientation in rigor and in the presence of nucleotide studied by electron spin resonance.
Myosin cross-bridge orientation in rigor and in the presence of nucleotide studied by electron spin resonance.
复制标题
通过电子自旋共振研究了严格的肌球蛋白跨桥方向和在核苷酸存在下的方向。
DOI：
10.1016/s0006-3495(89)82700-6
复制
发表时间：
1989
影响因子：
3.4
通讯作者：
Burghardt,TP
中科院分区：
文献类型：
--
作者：
Ajtai,K;French,AR;Burghardt,TP
用自旋标记的肌球蛋白亚片段1（S1）修饰肌纤维，在僵硬状态下和MgADP存在下测量肌纤维的倾斜系列电子自旋共振（ESR）谱。ESR谱是在静态磁场的低幅度调制下测量的，以确保发生最小的谱线形状失真。10个倾斜系列ESR数据集通过随附论文中描述的模型独立方法同时拟合（Burghardt，T. P.，和A. R.法语，1989年。Biophys. J. 56：525-534）。用这种方法计算了所研究的光纤的两种状态下探针角分布的序参量的平均值和平均值的标准误差。平均阶参数被用来重建探头的角度分布在两个维度，一个角度维度对应于一个极角测量相对于光纤轴，和其他的扭转角自由度的探头。我们发现，探针的角度分布的严格和MgADP状态的纤维不同，严格的分布是更广泛的，并转移相对于在MgADP的存在下的分布。严格分布的形状表明存在两个探针方向，一个类似于在MgADP的存在下，另一个在不同的方向。在MgADP存在下的分布的形状表明，结合的核苷酸的严谨的交叉桥转移到一个更均匀的一个通过引起交叉桥旋转的自旋人口。
The tilt series electron spin resonance (ESR) spectrum from muscle fibers decorated with spin labeled myosin subfragment 1 (S1) was measured from fibers in rigor and in the presence of MgADP. ESR spectra were measured at low amplitude modulation of the static magnetic field to insure that a minimum of spectral lineshape distortion occurs. Ten tilt series ESR data sets were fitted simultaneously by the model-independent methodology described in the accompanying paper (Burghardt, T. P., and A. R. French, 1989. Biophys. J. 56:525–534). By this method the average and standard error in the mean of order parameters for the probe angular distribution were calculated for the two states of the fiber investigated. The average order parameters were used to reconstruct the probe angular distribution in two dimensions, one angular dimension corresponding to a polar angle measured relative to the fiber axis, and the other a torsional angular degree of freedom of the probe. We find that the probe angular distributions for the rigor and MgADP states of the fiber differ such that the rigor distribution is broader and shifted relative to the distribution in the presence of MgADP. The shape of the rigor distribution suggests the presence of two probe orientations, one similar to that in the presence of MgADP, and another at a different orientation. The shape of the distribution in the presence of MgADP suggests that the binding of the nucleotide to the rigor cross-bridge shifts the spin population into a more homogeneous one by causing a cross-bridge rotation.
登录
查看更多内容
DOI：
10.1073/pnas.76.12.6346
发表时间：
1979-01-01
影响因子：
11.1
作者：
BOREJDO, J;PUTNAM, S;MORALES, MF
通讯作者：
MORALES, MF
与模型无关的电子自旋共振，用于测量生物组件中固定组件的顺序。
影响因子：
3.4
作者：
Burghardt,TP;Thompson,NL
通讯作者：
Thompson,NL
观察甘油肌纤维中严格的横桥的两个方向。
影响因子：
2.9
作者：
Ajtai,K;Burghardt,TP
通讯作者：
Burghardt,TP
肌肉横桥 MgADP 状态的探针研究：来自 1,5-IAEDANS 标记的肌球蛋白亚片段 1 装饰肌纤维的微观和波长依赖性荧光偏振。
影响因子：
2.9
作者：
Ajtai,K;Burghardt,TP
通讯作者：
Burghardt,TP
DOI：
10.1111/j.1432-1033.1967.tb00120.x
发表时间：
1967-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
BARANY, M;CONOVER, TE;GOFFART, M
通讯作者：
GOFFART, M