Major tyrosine identity determinants in Methanococcus jannaschii and Saccharomyces cerevisiae tRNA(Tyr) are conserved but expressed differently.
Major tyrosine identity determinants in Methanococcus jannaschii and Saccharomyces cerevisiae tRNA(Tyr) are conserved but expressed differently.
复制标题
詹氏甲烷球菌和酿酒酵母 tRNA(Tyr) 中的主要酪氨酸特性决定簇是保守的，但表达方式不同。
DOI：
--
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Richard Giegé
中科院分区：
文献类型：
--
作者：
Pierre Fechter;J. Rudinger;Mikhail A. Tukalo;Richard Giegé
使用体外 tRNA 转录本和小螺旋表明，来自古细菌詹氏甲烷球菌的酪氨酰-tRNA 合成酶 (TyrRS) 充电的 tRNA 的酪氨酸身份是由六个核苷酸决定的：鉴别器碱基 A73 和受体茎中的第一个碱基对 C1-G72 以及反密码子三联体。然而，反密码子残基仅微弱地参与身份确定，尤其是残基 35 和 36。上述身份集的完整性通过其转移到几个 tRNA 中得到验证，然后这些 tRNA 变得与来自 M. jannaschii 的野生型转录物一样有效地酪氨酸化。对 M. jannaschii 和酵母 tRNA(Tyr) 转录本的结构和酪氨酰化特性的温度依赖性实验表明，古菌转录本比酵母转录本具有更高的结构稳定性和增强的氨酰化行为。将 M. jannaschii 中的酪氨酸特性与酵母中的酪氨酸特性进行比较，两种生物体中主要决定簇（即 C1-G72 对）的保守性为古菌和真核氨酰化系统之间存在功能联系提供了额外的支持。
Using in vitro tRNA transcripts and minihelices it was shown that the tyrosine identity for tRNA charging by tyrosyl-tRNA synthetase (TyrRS) from the archaeon Methanococcus jannaschii is determined by six nucleotides: the discriminator base A73 and the first base-pair C1-G72 in the acceptor stem together with the anticodon triplet. The anticodon residues however, participate only weakly in identity determination, especially residues 35 and 36. The completeness of the aforementioned identity set was verified by its tranfer into several tRNAs which then become as efficiently tyrosylatable as the wild-type transcript from M. jannaschii. Temperature dependence experiments on both the structure and the tyrosylation properties of M. jannaschii and yeast tRNA(Tyr) transcripts show that the archaeal transcript has greater structural stability and enhanced aminoacylation behaviour than the yeast transcript. Tyrosine identity in M. jannaschii is compared to that in yeast, and the conservation of the major determinant in both organisms, namely the C1-G72 pair, gives additional support to the existence of a functional connection between archaeal and eukaryotic aminoacylation systems.
大肠杆菌起始子 tRNA 突变体，可抑制酿酒酵母中的琥珀密码子，并被酵母提取物用酪氨酸氨酰化。
DOI：
10.1073/pnas.88.24.11378
发表时间：
1991
影响因子：
11.1
作者：
Lee,CP;RajBhandary,UL
通讯作者：
RajBhandary,UL
影响因子：
5.6
作者：
Tocchini-Valentini, G;Saks, ME;Abelson, J
通讯作者：
Abelson, J
影响因子：
14.9
作者：
MILLIGAN, JF;GROEBE, DR;UHLENBECK, OC
通讯作者：
UHLENBECK, OC