Dentin regeneration by dental pulp stem cell therapy with recombinant human bone morphogenetic protein 2
Dentin regeneration by dental pulp stem cell therapy with recombinant human bone morphogenetic protein 2
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DOI：
10.1177/154405910408300802
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发表时间：
2004-08-01
影响因子：
7.6
通讯作者：
Akamine, A
中科院分区：
文献类型：
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作者：
Iohara, K;Nakashima, M;Akamine, A
再生医学是以干细胞、信号和支架为基础的。牙髓组织有可能在有害刺激下再生牙本质，如龋齿。祖细胞/干细胞负责这种再生。因此，干细胞疗法在牙本质再生方面有相当大的前景。猪牙髓细胞作为三维颗粒培养，与单层相比，可促进成牙本质细胞分化。牙本质涎磷蛋白(Dspp)和成釉蛋白/基质金属蛋白酶20(MMP20)的表达证实了牙髓细胞向成牙本质细胞分化，并受到形态发生信号骨形态发生蛋白2(BMP2)的刺激。在体外实验的基础上，对狗的牙髓前体/干细胞进行了体内评价。将经BMP2处理的颗粒培养物自体移植到被切断的牙髓上，刺激修复性牙本质的形成。综上所述，BMP2可以引导牙髓前体/干细胞分化为成牙本质细胞，并导致牙本质的形成。
Regenerative medicine is based on stem cells, signals, and scaffolds. Dental pulp tissue has the potential to regenerate dentin in response to noxious stimuli, such as caries. The progenitor/stem cells are responsible for this regeneration. Thus, stem cell therapy has considerable promise in dentin regeneration. Culture of porcine pulp cells, as a three-dimensional pellet, promoted odontoblast differentiation compared with monolayers. The expression of dentin sialophosphoprotein ( Dspp) and enamelysin/matrix metalloproteinase 20 (MMP20) mRNA confirmed the differentiation of pulp cells into odontoblasts and was stimulated by the morphogenetic signal, bone morphogenetic protein 2 (BMP2). Based on the in vitro experiments, an in vivo evaluation of pulp progenitor/stem cells in the dog was performed. The autogenous transplantation of the BMP2-treated pellet culture onto the amputated pulp stimulated reparative dentin formation. In conclusion, BMP2 can direct pulp progenitor/stem cell differentiation into odontoblasts and result in dentin formation.