Shugoshin enables tension-generating attachment of kinetochores by loading Aurora to centromeres
Shugoshin enables tension-generating attachment of kinetochores by loading Aurora to centromeres
复制标题
DOI：
10.1101/gad.1497307
复制
发表时间：
2007-02-15
影响因子：
10.5
通讯作者：
Watanabe, Yoshinori
中科院分区：
文献类型：
--
作者：
Kawashima, Shigehiro A.;Tsukahara, Tatsuya;Watanabe, Yoshinori
裂变酵母shugoshin Sgo 1是减数分裂特异性的，并与蛋白磷酸酶2A合作，以保护减数分裂I的着丝粒粘着蛋白。另一种shugoshin样蛋白Sgo 2需要异染色质蛋白Swi 6/HP 1才能完全存活，它在有丝分裂和减数分裂中对染色体分离起着至关重要的作用;然而，其潜在机制完全难以捉摸。我们在这里证明，与Sgo 1，Sgo 2是cytomerous的保护粘连蛋白。相反，Sgo 2与Bir 1/Survivin相互作用，促进Aurora激酶复合物定位于着丝粒周围区域，以纠正着丝粒的错误附着，从而使张力产生附着成为可能。强迫本地化的Bir 1的着丝粒部分恢复的缺陷sgo 2 δ。这种新发现的shugoshin与Survivin的相互作用在有丝分裂和减数分裂之间是保守的，并且可能在真核生物中是保守的。我们建议，确保双极附着的动粒是shugoshin的主要作用和凝聚力保护的作用可能共同发展，以促进这一进程。
Fission yeast shugoshin Sgo1 is meiosis specific and cooperates with protein phosphatase 2A to protect centromeric cohesin at meiosis I. The other shugoshin-like protein Sgo2, which requires the heterochromatin protein Swi6/HP1 for full viability, plays a crucial role for proper chromosome segregation at both mitosis and meiosis; however, the underlying mechanisms are totally elusive. We here demonstrate that, unlike Sgo1, Sgo2 is dispensable for centromeric protection of cohesin. Instead, Sgo2 interacts with Bir1/Survivin and promotes Aurora kinase complex localization to the pericentromeric region, to correct erroneous attachment of kinetochores and thereby enable tension-generating attachment. Forced localization of Bir1 to centromeres partly restored the defects of sgo2 Delta. This newly identified interaction of shugoshin with Survivin is conserved between mitosis and meiosis and presumably across eukaryotes. We propose that ensuring bipolar attachment of kinetochores is the primary role of shugoshin and the role of cohesion protection might have codeveloped to facilitate this process.