MATERNAL INHERITANCE OF THE MOUSE MITOCHONDRIAL GENOME IS NOT MEDIATED BY A LOSS OR GROSS ALTERATION OF THE PATERNAL MITOCHONDRIAL-DNA OR BY METHYLATION OF THE OOCYTE MITOCHONDRIAL-DNA
MATERNAL INHERITANCE OF THE MOUSE MITOCHONDRIAL GENOME IS NOT MEDIATED BY A LOSS OR GROSS ALTERATION OF THE PATERNAL MITOCHONDRIAL-DNA OR BY METHYLATION OF THE OOCYTE MITOCHONDRIAL-DNA
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DOI：
10.1016/0012-1606(84)90210-0
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发表时间：
1984-01-01
影响因子：
2.7
通讯作者：
HO, SM
中科院分区：
文献类型：
--
作者：
HECHT, NB;LIEM, H;HO, SM
为了评估是否存在或修改父亲的线粒体DNA或甲基化的卵母细胞线粒体DNA可能是母系遗传的线粒体在哺乳动物中的分子基础，线粒体基因组进行了分析，在4个减数分裂和减数分裂后的睾丸细胞类型和卵母细胞从小鼠。包括精子在内的所有睾丸细胞类型都含有线粒体DNA。在减数分裂和精子发生结束之间，每个单倍体基因组的线粒体基因组的数量减少8至10倍，其中精子含有apprx。每个线粒体1个线粒体基因组拷贝。用6种不同酶进行限制性内切酶消化表明，来自减数分裂细胞、早期单倍体细胞、晚期单倍体细胞和精子的睾丸线粒体DNA的DNA序列没有明显差异。根据异源异构体MspI和HpaII的差异消化标准，线粒体DNA在精子发生过程中没有差异甲基化。用7种甲基化敏感的限制性内切酶消化精子和卵母细胞的线粒体DNA，未发现甲基化差异。
To evaluate whether the absence or modification of paternal mitochondrial DNA or methylation of the oocyte mitochondria DNA could be the molecular basis for maternal inheritance of mitochondria in mammals, the mitochondrial genome was analyzed in 4 meiotic and postmeiotic testicular cell types and in oocytes from the mouse. All testicular cell types including spermatozoa contain mitochondrial DNA. Between meiosis and the end of spermatogenesis the number of mitochondrial genomes per haploid genome decreases 8- to 10-fold with spermatozoa containing .apprx. 1 copy of the mitochondrial genome per mitochondrion. Restriction enzyme digestions with 6 different enzymes indicate no gross differences in DNA sequence in the testicular mitochondrial DNA from meiotic cells, early haploid cells, late haploid cells and spermatozoa. By the criterion of differential digestion with the isoschizomers, MspI and HpaII, the mitochondrial DNA is not differentially methylated during spermatogenesis. No methylation differences were detected in mitochondrial DNA from sperm and oocytes following digestion with 7 methylation-sensitive restriction enzymes.