Improving sensitivity in nonlinear Raman microspectroscopy imaging and sensing
Improving sensitivity in nonlinear Raman microspectroscopy imaging and sensing
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DOI：
10.1117/1.3533317
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发表时间：
2011-02-01
影响因子：
3.5
通讯作者：
Yakovlev, Vladislav V.
中科院分区：
文献类型：
--
作者：
Arora, Rajan;Petrov, Georgi I.;Yakovlev, Vladislav V.
基于宽带相干反斯托克斯拉曼散射的非线性拉曼光谱是一种新兴的非侵入性、化学特异性、显微分析组织和大量细胞和粒子的技术。这种成像的敏感性是许多拟议的生物医学应用的关键方面。结果表明，入射激光功率是控制这种灵敏度的主要参数。通过对激光系统的精心优化，获得高质量的多kHz频率振动光谱成为可能。(C)2011年，光学仪器工程师学会。[DOI：10.1117/1.3533317]
Nonlinear Raman microspectroscopy based on a broadband coherent anti-Stokes Raman scattering is an emerging technique for noninvasive, chemically specific, microscopic analysis of tissues and large population of cells and particles. The sensitivity of this imaging is a critical aspect of a number of the proposed biomedical application. It is shown that the incident laser power is the major parameter controlling this sensitivity. By careful optimizing the laser system, the high-quality vibrational spectra acquisition at the multi-kHz rate becomes feasible. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI:10.1117/1.3533317]