PRP19: a novel spliceosomal component.
PRP19: a novel spliceosomal component.
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PRP19：一种新型剪接体成分。
DOI：
10.1128/mcb.13.3.1876-1882.1993
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发表时间：
1993
影响因子：
5.3
通讯作者：
Abelson,J
中科院分区：
文献类型：
--
作者：
Cheng,SC;Tarn,WY;Tsao,TY;Abelson,J
我们通过互补酿酒酵母prp 19突变体的温度敏感性生长缺陷，分离了剪接因子PRP19的基因。该基因编码一种分子量为56，500的502个氨基酸残基的蛋白质，与数据库中的序列没有同源性。与其他PRP蛋白或哺乳动物剪接因子不同，PRP 19的序列没有可识别的基序。免疫沉淀研究表明，PRP19在剪接反应过程中与剪接体相关。虽然PRP 19的确切功能仍然未知，但PRP 19似乎与其他PRP蛋白或其他剪接体组分不同。
We have isolated the gene of a splicing factor, PRP19, by complementation of the temperature-sensitive growth defect of the prp19 mutant ofSaccharomyces cerevisiae. The gene encodes a protein of 502 amino acid residues of molecular weight 56,500, with no homology to sequences in the data base. Unlike other PRP proteins or mammalian splicing factors, the sequence of PRP19 has no discernible motif. Immunoprecipitation studies showed that PRP19 is associated with the spliceosome during the splicing reaction. Although the exact function of PRP19 remains unknown, PRP19 appears to be distinct from the other PRP proteins or other spliceosomal components.