Loss of the extraembryonic ectoderm in Elf5 mutants leads to defects in embryonic patterning
Loss of the extraembryonic ectoderm in Elf5 mutants leads to defects in embryonic patterning
复制标题
DOI：
10.1242/dev.01819
复制
发表时间：
2005-05-01
期刊：
影响因子：
4.6
通讯作者：
Pfeffer, PL
中科院分区：
文献类型：
--
作者：
Donnison, M;Beaton, A;Pfeffer, PL
胚胎外胚层(Exe)对于哺乳动物胎盘的形成和胚胎在子宫内的存活是必不可少的。我们通过靶向缺失转录因子ELF5获得了一种缺失EXE的小鼠模型。虽然ELF5突变胚胎植入并形成外胎盘锥体，但无法获得滋养层干细胞，这表明EXE的缺失是TS细胞缺乏维持的结果。没有EXE组织的胚胎能够形成前内脏内胚层，但不能进行原肠形成，这表明在特定的发育窗口中，EXE在胚胎模式形成中起着至关重要的作用。
The extraembryonic ectoderm (ExE) is essential for mammalian placental formation and survival of the embryo in utero. We have obtained a mouse model lacking the ExE, by targeted deletion of the transcription factor Elf5. Although Elf5 mutant embryos implant and form an ectoplacental cone, no trophoblast stem (TS) cells can be derived, indicating that the absence of ExE is a result of the lack of TS cell maintenance. Embryos without ExE tissue are able to form the anterior visceral endoderm but fail to undergo gastrulation, demonstrating an essential role for the ExE in embryonic patterning during a defined window of development.