cDNA libraries from human tissues and cell lines.
cDNA libraries from human tissues and cell lines.
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来自人体组织和细胞系的 cDNA 文库。
DOI：
10.1159/000133595
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Xu,J
中科院分区：
文献类型：
--
作者：
Swaroop,A;Xu,J
我们报道了在λ噬菌体载体（Charon BS，Λ gt 11，ΛSHK，或ΛzapII）中从人组织和细胞系构建的34个cDNA文库。使用oligo-dT作为引物，从聚A+RNA合成cDNA，并在连接到载体臂之前进行大小选择。来自人类组织和细胞系的高复杂性cDNA文库将是基因组作图研究和疾病基因鉴定的宝贵资源。
We report the construction of 34 cDNA libraries from human tissues and cell lines in lambda phage vectors (Charon BS, Λgt11, ΛSHK, or ΛzapII). The cDNA was synthesized from poly A+RNA, using oligo-dT as a primer, and size-selected before ligation to vector arms. High-complexity cDNA libraries from human tissues and cell lines should be a valuable resource for genome mapping studies and identification of disease genes.