Unique adenosine 3',5' cyclic monophosphate phosphodiesterase messenger ribonucleic acids in rat spermatogenic cells: evidence for differential gene expression during spermatogenesis.
Unique adenosine 3',5' cyclic monophosphate phosphodiesterase messenger ribonucleic acids in rat spermatogenic cells: evidence for differential gene expression during spermatogenesis.
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大鼠生精细胞中独特的腺苷 3,5 环单磷酸磷酸二酯酶信使核糖核酸：精子发生过程中差异基因表达的证据。
DOI：
10.1095/biolreprod46.6.1027
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发表时间：
1992
影响因子：
3.6
通讯作者：
Conti,M
中科院分区：
文献类型：
--
作者：
Welch,JE;Swinnen,JV;O'Brien,DA;Eddy,EM;Conti,M
四个cAMP磷酸二酯酶(cAMP-PDE)基因(ratPDE1、ratPDE1、ratPDE3和ratPDE4)在大鼠睾丸中表达(Swinnen等人，PNAS USA 1989；86：5325)。由于在雄性生殖细胞中存在多聚体PDE1mRNAs和RatPDE1mRNAs，用纯化的生精细胞群体研究了它们的发育表达，发现RatPDE1mRNAs(4.0和2.8kb)在粗线期精子细胞中含量丰富，RatPDE1mRNAs在圆形精细胞中表达降低，在凝聚的精细胞/残体中缺失。而PDE1mRNAs(4.0、3.5、3.1、2.8和2.4kb)在圆形精细胞中含量丰富，在浓缩的精细胞/残体中含量较低。小鼠圆形精子细胞中也存在与toratPDE1相关的转录本。生殖细胞胞浆层析鉴定出cAMP-PDE活性的两个峰。A峰在所有的生殖细胞群中都很明显，B峰在粗线型精母细胞和圆形精细胞中出现，但在浓缩的精细胞/残余体中处于检测范围内。凝聚的精细胞/残体中B峰活性的大幅下降可能与精子发生过程中观察到的PDE1mRNA水平的下降有关。凝聚的精细胞/残体中持续的A峰活性与这些细胞中的rPDE1mRNA的存在相吻合，这表明PDE1酶可能在精子发生和精子中发挥作用。
Four cAMP phosphodiesterase (cAMP-PDE) genes (ratPDE1,ratPDE1,ratPDE3, andratPDE4) are expressed in the rat testis (Swinnen et al., PNAS USA 1989; 86:5325). Since multipleratPDE1andratPDE1mRNAs were present in male germ cells, their developmental expression was investigated by using purified spermatogenic cell populations.RatPDE1mRNAs (4.0 and 2.8 kb) were found to be abundant in pachytene spermatocytes.RatPDE1mRNA levels were decreased in round spermatids and absent from condensing spermatids/residual bodies. However, multipleratPDE1mRNAs (4.0, 3.5, 3.1, 2.8, and 2.4 kb) were abundant in round spermatids, and lower amounts were present in condensing spermatids/residual bodies. Transcripts related toratPDE1were also present in mouse round spermatids. Chromatography of germ cell cytosol identified two peaks of cAMP-PDE activity. Whereas peak A was evident in all germ cell populations examined, peak B was present in pachytene spermatocytes and round spermatids, but was at the limit of detection in condensing spermatids/residual bodies. The large decrease in peak B activity in condensing spermatids/residual bodies may be related to the drop inratPDE1mRNA levels observed during spermatogenesis. The sustained peak A activity in condensing spermatids/residual bodies coincides with the presence ofratPDE1mRNA in these cells and suggests that theratPDE1enzyme may function during spermiogenesis and in spermatozoa.