Isolated lymphatic vessel lumen perfusion system for assessing nanomaterial movements and nanomaterial-induced responses in lymphatic vessels
Isolated lymphatic vessel lumen perfusion system for assessing nanomaterial movements and nanomaterial-induced responses in lymphatic vessels
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DOI：
10.1016/j.nantod.2020.101018
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发表时间：
2021-02
期刊：
影响因子：
17.4
通讯作者：
C. Kuroda;Kumiko Ajima;Katsuya Ueda;Atsushi Sobajima;Kazushige Yoshida;Takayuki Kamanaka;Jun Sasaki;Haruka Ishida;H. Haniu;M. Okamoto;Kaoru Aoki;Hiroyuki Kato;N. Saito
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文献类型：
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作者：
C. Kuroda;Kumiko Ajima;Katsuya Ueda;Atsushi Sobajima;Kazushige Yoshida;Takayuki Kamanaka;Jun Sasaki;Haruka Ishida;H. Haniu;M. Okamoto;Kaoru Aoki;Hiroyuki Kato;N. Saito
纳米材料最近被广泛研究用于各种各样的生物应用，包括药物递送、生物成像和再生医学。然而，由于缺乏评估其在活生物体中的安全性的既定方法，它们几乎没有临床用途。特别是，关于纳米材料的生物活性仍有许多未知之处。纳米材料可以通过静脉内给药，并通过血流到达各种组织，或直接注射到皮下组织或病变，如肿瘤。已知这些纳米材料会进入淋巴管，迁移到淋巴系统，并在淋巴结中积聚。因此，为了阐明它们的生物学特性，重要的是要阐明它们与淋巴管的相互作用。然而，人们对它们在淋巴管中的运动和对淋巴管的影响知之甚少。在这项研究中，我们开发并评估了离体淋巴管腔灌注系统，使我们能够直观和定量地阐明纳米材料和淋巴管之间的相互作用，从而阐明纳米材料的生物安全性。从活体大鼠中取出血管并在体外进行研究。与体内检查相比，这种灌注系统可以更高分辨率，更详细地检查纳米材料的运动以及淋巴管对这种运动的反应。此外，新系统还可以对单个淋巴管对纳米材料的生理反应进行定量和组织学评估。使用该系统提供有关纳米材料生物学的基本信息，预计将大大有助于开发各种各样的纳米材料的生物应用。
Nanomaterials have recently been investigated extensively for a wide variety of biological applications, including drug delivery, bioimaging, and regenerative medicine. However, they have found little clinical use because of the lack of an established method for assessing their safety in living organisms. In particular, much remains unknown about the biokinetics of nanomaterials. Nanomaterials may be administered intravenously and reach various tissues via the bloodstream or injected directly into subcutaneous tissue or lesions such as tumors. These nanomaterials are known to enter lymphatic vessels, migrate to the lymph system, and accumulate in lymph nodes. To clarify their biokinetics, therefore, importance should be placed on elucidating their interactions with lymphatic vessels. However, little is known about their movements in, and influence on, the lymphatic vessels. In this study, we developed and assessed the isolated lymphatic vessel lumen perfusion system to allow us to visually and quantitatively elucidate the interactions between nanomaterials and lymphatic vessels, and thereby the biosafety of nanomaterials. The vessel is removed from a living rat and studied in vitro. This perfusion system, compared to in vivo examinations, allows higher-resolution, more detailed examination of the movement of nanomaterials and the lymphatic vessel’s reaction to this movement. In addition, the new system also enables both quantitative and histological assessment of a single lymphatic vessel’s physiological reaction to nanomaterials. Use of this system to provide essential information about nanomaterial biokinetics is expected to contribute significantly to the development of a wide variety of biological applications of nanomaterials.