RpoE is a Putative Antibiotic Resistance Regulator of Salmonella enteric Serovar Typhi.
RpoE is a Putative Antibiotic Resistance Regulator of Salmonella enteric Serovar Typhi.
复制标题
RpoE 是一种公认的肠伤寒沙门氏菌抗生素耐药性调节剂。
DOI：
10.1007/s00284-015-0983-7
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Du Hong（杜鸿）
中科院分区：
文献类型：
--
作者：
Xie Xiaofang;Zhang Haifang;Zheng Yi;Li Aiqing;Wang Min;Zhou Huiqin;Zhu Xueming;Schneider Zachary;Chen Liang;Kreiswirth Barry N;Du Hong（杜鸿）
细菌抗微生物药物耐药性与编码外排泵基因的上调和编码外膜蛋白（OMPs）基因的下调有关。细菌的基因表达主要由RpoE等sigma因子（σ因子）启动，在应对多种环境胁迫中起着重要作用。在这里，我们报告了首次观察到RpoE在肠血清型伤寒沙门氏菌（S.Typhi）中作为抗生素耐药性调节剂。在本研究中，我们发现s。伤寒杆菌GIFU10007对几种抗菌剂的耐药性升高，包括β-内酰胺类、喹诺酮类和氨基糖苷类。基因组DNA微阵列分析用于研究野生型和突变体对氨苄西林的差异基因表达谱。结果表明，在ΔrpoEversus野生型菌株中，共有57个基因出现差异表达（增加或减少2倍）。在ΔrpoE中，两个外膜蛋白基因ompFandompC的表达量显著下调（分别比野生型菌株低6倍和7倍），在ΔrpoE中，外排泵AraC/XylS家族成员RamA的表达量上调约4倍。我们的研究结果表明，RpoE是一种潜在的抗菌调节剂。伤寒，控制OMP基因的下调和外排系统的上调。
Bacterial antimicrobial resistance has been associated with the up regulation of genes encoding efflux pumps and the down regulation of genes encoding outer membrane proteins (OMPs). Gene expression in bacteria is primarily initiated by sigma factors (σ factors) such as RpoE, which plays an important role in responding to many environmental stresses. Here, we report the first observation that RpoE serves as an antibiotic resistance regulator inSalmonella entericserovar Typhi (S.Typhi). In this study, we found that therpoEmutant (ΔrpoE) ofS.Typhi GIFU10007 has elevated resistance to several antimicrobial agents, including β-lactams, quinolones, and aminoglycosides. Genomic DNA microarray analysis was used to investigate the differential gene expression profiles between a wild type andrpoEmutant in response to ampicillin. The results showed that a total of 57 genes displayed differential expression (two-fold increase or decrease) in ΔrpoEversus the wild-type strain. The expressions of two outer membrane protein genes,ompFandompC, were significantly down-regulated in ΔrpoE(six and seven-fold lower in comparison to wild-type strain) and RamA, a member of the efflux pump AraC/XylS family, was up-regulated about four-fold in the ΔrpoE. Our results suggest RpoE is a potential antimicrobial regulator inS.Typhi, controlling both the down regulation of the OMP genes and up-regulating the efflux system.