Interaction of Aurora-A and centrosomin at the microtubule-nucleating site in Drosophila and mammalian cells.
Interaction of Aurora-A and centrosomin at the microtubule-nucleating site in Drosophila and mammalian cells.
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DOI：
10.1083/jcb.200305048
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发表时间：
2003-09-01
期刊：
影响因子：
--
通讯作者：
Kuriyama R
中科院分区：
文献类型：
--
作者：
Terada Y;Uetake Y;Kuriyama R
A mitosis-specific Aurora-A kinase has been implicated in microtubule organization and spindle assembly in diverse organisms. However, exactly how Aurora-A controls the microtubule nucleation onto centrosomes is unknown. Here, we show that Aurora-A specifically binds to the COOH-terminal domain of a Drosophila centrosomal protein, centrosomin (CNN), which has been shown to be important for assembly of mitotic spindles and spindle poles. Aurora-A and CNN are mutually dependent for localization at spindle poles, which is required for proper targeting of γ-tubulin and other centrosomal components to the centrosome. The NH2-terminal half of CNN interacts with γ-tubulin, and induces cytoplasmic foci that can initiate microtubule nucleation in vivo and in vitro in both Drosophila and mammalian cells. These results suggest that Aurora-A regulates centrosome assembly by controlling the CNN's ability to targeting and/or anchoring γ-tubulin to the centrosome and organizing microtubule-nucleating sites via its interaction with the COOH-terminal sequence of CNN.
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发表时间：
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期刊：
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