Increased interferon-γ, interleukin-12p40 and IL-8 production in Propionibacterium acnes-treated peripheral blood mononuclear cells from patient with acne vulgaris Host response but not bacterial species is the determinant factor of the disease
Increased interferon-γ, interleukin-12p40 and IL-8 production in Propionibacterium acnes-treated peripheral blood mononuclear cells from patient with acne vulgaris Host response but not bacterial species is the determinant factor of the disease
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DOI：
10.1016/j.jdermsci.2009.02.015
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发表时间：
2009-07-01
影响因子：
4.6
通讯作者：
Mizutani, Hitoshi
中科院分区：
文献类型：
--
作者：
Sugisaki, Hitomi;Yamanaka, Keiichi;Mizutani, Hitoshi
【摘要】：背景:寻常痤疮是一种常见于青春期的面部和躯干皮脂囊的多因素炎症性疾病。最初，痤疮是一种非炎症性粉刺。随后，炎症反应演变成脓疱、肉芽肿和囊性病变。已提出许多致病机制，包括皮脂排泄、毛囊阻塞、毛发上皮角化受损、细菌过度生长和免疫机制；痤疮丙酸杆菌（P. acnes）的作用尤为重要。兼性厌氧革兰氏阳性杆与痤疮发病机制有关。然而，宿主对痤疮丙酸杆菌的免疫反应尚未阐明。目的：本研究的目的是评估痤疮丙酸杆菌免疫反应的重要性以及痤疮发病机制中的细菌学因素。方法：培养从痤疮皮损和健康志愿者皮肤中分离的痤疮丙酸杆菌。用活痤疮丙酸杆菌刺激痤疮患者或健康志愿者的外周血单核细胞 (PBMC)，并使用 RT-PCR 和 ELISA 评估细胞因子的产生。结果：与正常供体相比，痤疮患者的 PBMC 中 IFN-γ、IL-12p40 和 IL-8 mRNA 和蛋白质的产生显着增加。然而，从痤疮皮损或正常受试者中分离出的不同痤疮丙酸杆菌物种显示，痤疮患者和正常受试者PBMC产生的细胞因子没有差异。结论：痤疮丙酸杆菌的炎症反应似乎归因于痤疮丙酸杆菌诱导的宿主免疫反应，而不是来自正常皮肤或痤疮皮损的痤疮丙酸杆菌菌株。 (C) 2009 年日本皮肤病研究学会。由爱思唯尔爱尔兰有限公司出版。保留所有权利。
Background: Acne vulgaris is a multifactorial inflammatory disease of the sebaceous follicles of the face and torso that frequently Occurs in adolescence. Initially, acne starts as a non-inflammatory comedo. Subsequently, inflammatory reactions evolve to pustules, granulomas and cystic lesions. Many pathogenic mechanisms have been proposed including sebum excretion, obstruction of hair follicles, impaired keratinization of hair epithelium, bacterial overgrowth and immunological mechanisms; the role of Propionibacterium acnes (P. acnes) is particularly important. Facultative anaerobic gram-positive rods have been implicated in acne pathogenesis. However, the host immune response to P. acnes has not been as yet elucidated.Objectives: The aim of the present Study is to evaluate the importance of the immune response to P. acnes and the bacteriological factor in the pathogenesis of acne.Methods: P. acnes isolated from acne lesions and healthy volunteers skin were Cultured. The peripheral blood mononuclear cells (PBMC) from acne patients or healthy volunteers were stimulated with viable P. acnes, and cytokine production was evaluated using RT-PCR and ELISA.Results: IFN-gamma, IL-12p40, and IL-8 mRNA and protein production were significantly increased in PBMC from acne patients compared to that from normal donors. However, different P. acnes species isolated from acne lesions or normal subjects showed no difference in cytokines production from acne patients and normal subjects PBMC.Conclusions: The inflammatory response of acne appears to be attributable to P. acnes-induced host immune response rather than P. acnes strains from normal skin or acne lesions. (C) 2009 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.