Lipid-modified conjugated polymer nanoparticles for cell imaging and transfection
Lipid-modified conjugated polymer nanoparticles for cell imaging and transfection
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DOI：
10.1039/b915112e
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发表时间：
2010-01-01
影响因子：
--
通讯作者：
Wang, Shu
中科院分区：
文献类型：
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作者：
Feng, Xuli;Tang, Yanli;Wang, Shu
制备了一种新型的基于脂质修饰的阳离子型聚芴基苯撑（PFPL）荧光纳米粒子。PFPL是两亲性的，并且具有两个独特的结构特征。首先，考虑到脂质是生物相容的并且可以容易地进入细胞质并且还为缀合的聚合物主链提供保护层的事实，将脂质设计为侧链。第二，铵侧基可以与提供PFPL作为基因递送载体的基因相互作用。PFPL在水中形成尺寸约为50 nm的均匀纳米颗粒。PFPL纳米颗粒具有优异的光稳定性和很小的细胞毒性。它可以很容易地通过胞吞作用进入细胞质（4小时内），并可用于细胞成像。此外，PFPL颗粒可以成功地将质粒（pCX-EGFP）递送到肺癌细胞（A549）中进行转录和翻译。因此，可以使用荧光显微镜直接跟踪质粒进入细胞和GFP蛋白的表达。PFPL纳米颗粒是一个很好的候选细胞成像和跟踪重要的细胞内过程在未来的研究。
A new fluorescent nanoparticle based on lipid-modified cationic poly(fluorenylene phenylene) (PFPL) was prepared and characterized. The PFPL is amphiphilic and has two unique structural features. First, the lipid is designed as a side chain in consideration of the fact that the lipid is biocompatible and can enter the cytoplasm easily and also provides protective layers to conjugated polymer backbones. Second, the ammonium pendant groups can interface with genes that offer PFPL as a vector for gene delivery. The PFPL forms uniform nanoparticles in water with a size of about 50 nm. The PFPL nanoparticle exhibits excellent photostability and little cytotoxicity. It can easily enter the cytoplasm via endocytosis (within 4 hours) and can be used for cell imaging. Furthermore, the PFPL particle can successfully deliver plasmids (pCX-EGFP) into lung cancer cells (A549) for transcription and translation. Thus, one can directly track the entrance of plasmids into cells and the expression of GFP proteins using fluorescence microscopy. The PFPL nanoparticle is a good candidate for cell imaging and tracking important intracellular processes in future research.