Micro-Gripper: A new concept for a monolithic single-cell manipulation device
Micro-Gripper: A new concept for a monolithic single-cell manipulation device
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DOI：
10.1016/j.sna.2015.10.024
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发表时间：
2015-12-01
影响因子：
4.6
通讯作者：
Dietzel, Andreas
中科院分区：
文献类型：
--
作者：
Garces-Schroeder, Mayra;Leester-Schaedel, Monika;Dietzel, Andreas
单细胞分析和操作方法对于基础研究以及实际应用（如体外受精（IVF）和药物研发）变得越来越重要。大量讨论夹持器、注射自动化或细胞固定化的出版物反映了对适当处理工具的需求。对现有装置的改进的努力集中在多个参数的连续监测上，例如夹持力、介质的构成或视频序列的记录，使得操作者或操作系统能够验证方法的适当功能和操作的成功。在这个概念研究中描述的方法统一的细胞定位，固定，工具对齐，操作和分析的步骤，通过将必要的设备集成到一个单一的单片工具的基础上的微机械夹具。新设计的关键机械和流体方面的研究分析计算和有限元分析，以评估微夹持器的可行性。在这样做的过程中，已经确定了卵母细胞内的局部临界应力，从而可以得出关于微夹持器设计的结论。此外，单片微制造路线进行了描述，并提出了集成的注射和固定化通道的工艺开发的第一个结果。(C)2015 Elsevier B.V.版权所有。
Single-cell analysis and manipulation methods are becoming increasingly important for fundamental research, as well as practical applications, such as in vitro fertilization (IVF) and pharmaceutical research and development. The need for adequate handling tools is reflected in a large number of publications discussing grippers, injection automation, or cell immobilization. Endeavors toward the improvement of existing devices focus on the continuous monitoring of multiple parameters, such as gripping force, constitution of the medium, or recording of video sequences, enabling the operator or the operating system to verify proper functioning of the method and the success of the operation. The approach described in this conceptual study unifies the steps of cell positioning, immobilization, tool alignment, manipulation, and analysis by integrating the necessary devices into a single monolithic tool based on a micromechanical gripper. The critical mechanical and fluidic aspects of the new design are investigated by analytical calculations and finite-element analysis to evaluate the feasibility of the micro-gripper. In doing so, local critical stresses inside the oocytes have been determined, allowing conclusions on the micro-gripper design. Furthermore, the monolithic micro fabrication route is described, and the first results of the process development for integrated injection and immobilization channels are presented. (C) 2015 Elsevier B.V. All rights reserved.