Preparation of highly magnetic chitosan particles and their use for affinity purification of enzymes
Preparation of highly magnetic chitosan particles and their use for affinity purification of enzymes
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DOI：
10.1002/jctb.820
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发表时间：
2003-05-01
影响因子：
3.4
通讯作者：
Zeng, HM
中科院分区：
文献类型：
--
作者：
An, XN;Su, ZX;Zeng, HM
一种新的协议，用于制备高磁性壳聚糖颗粒，具有高达3500 Oe的矫顽力，已经开发。颗粒的表面用醛基官能化以促进亲和配体的附接。研究了高磁性壳聚糖微粒的制备及磁性壳聚糖微粒固定化胰蛋白酶的最佳条件，并将磁性壳聚糖微粒用于亲和纯化抑肽酶，对亲和纯化条件进行了详细讨论。该方法已成功应用于抑肽酶的亲和纯化。(C)2003化学工业协会。
A novel protocol for preparation of highly magnetic chitosan particles, with a coercive force as high as 3500 Oe, has been developed. The surface of the particles was functionalized with aldehyde groups to facilitate the attachment of affinity ligands. The optimum conditions for the preparation of highly magnetic chitosan particles and immobilization of trypsin on magnetic particles were obtained; these particles were then used for affinity purification of aprotinin, and the conditions of affinity purification are discussed in detail. The proposed method was successfully applied to the affinity purification of aprotinin. (C) 2003 Society of Chemical Industry.