Cell adhesion and long-term survival of transplanted mesenchymal stem cells: a prerequisite for cell therapy.
Cell adhesion and long-term survival of transplanted mesenchymal stem cells: a prerequisite for cell therapy.
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DOI：
10.1155/2015/632902
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发表时间：
2015
影响因子：
--
通讯作者：
Hwang KC
中科院分区：
文献类型：
--
作者：
Lee S;Choi E;Cha MJ;Hwang KC
大量文献表明，间充质干细胞(MSCs)具有自我更新能力和广泛的分化潜能，是一种有吸引力的治疗资源，在损伤组织的再生中具有良好的作用。尽管MSCs的治疗潜力已在临床前和临床研究中得到证实，但仍有几个问题尚未解决。骨髓间充质干细胞在临床应用的一个主要限制因素是，由于恶劣的微环境和细胞黏附能力的丧失，它们在损伤部位的存活能力很差。为了提高移植的MSCs的存活率，已开发出调控细胞凋亡信号和增强细胞黏附的策略，如细胞因子、生长因子和抗凋亡分子的预处理、遗传修饰和低氧预适应。为了成功地应用于临床，需要更合适的动物模型和对骨髓间充质干细胞治疗机制的更多了解。然而，使用骨髓间充质干细胞的干细胞疗法的发展具有治疗退行性疾病的潜力。该综述讨论了通过抑制失巢凋亡来提高MSC存活率的各种方法。
The literature provides abundant evidence that mesenchymal stem cells (MSCs) are an attractive resource for therapeutics and have beneficial effects in regenerating injured tissues due to their self-renewal ability and broad differentiation potential. Although the therapeutic potential of MSCs has been proven in both preclinical and clinical studies, several questions have not yet been addressed. A major limitation to the use of MSCs in clinical applications is their poor viability at the site of injury due to the harsh microenvironment and to anoikis driven by the loss of cell adhesion. To improve the survival of the transplanted MSCs, strategies to regulate apoptotic signaling and enhance cell adhesion have been developed, such as pretreatment with cytokines, growth factors, and antiapoptotic molecules, genetic modifications, and hypoxic preconditioning. More appropriate animal models and a greater understanding of the therapeutic mechanisms of MSCs will be required for their successful clinical application. Nevertheless, the development of stem cell therapies using MSCs has the potential to treat degenerative diseases. This review discusses various approaches to improving MSC survival by inhibiting anoikis.