Transcriptome and metabolome analyses reveal the efficiency of in vitro regeneration by TDZ pretreatment in mulberry
Transcriptome and metabolome analyses reveal the efficiency of in vitro regeneration by TDZ pretreatment in mulberry
复制标题
DOI：
10.1016/j.scienta.2022.111678
复制
发表时间：
2022-11-25
影响因子：
4.3
通讯作者：
He, Ningjia
中科院分区：
文献类型：
--
作者：
Luo, Yiwei;Han, Yuanxiang;He, Ningjia
噻二唑(Thidiazuron，TDZ)是一种植物生长调节剂，被广泛用作落叶剂和细胞分裂素类化合物。此外，桑树也是一种重要的经济作物。然而，在大多数桑树物种中，再生是困难的，控制再生过程的调控机制尚不清楚。本研究通过TDZ的应用，建立了一套高效的桑树不定芽再生体系。结果表明，四倍体诺塔比兰的最佳不定芽诱导率为70.83%。川北1号(CB1)和黄曲霉96.82%。云花(YH)是以叶柄叶片为外植体，经芽前处理获得的。对经TDZ(T8)和对照(CK)处理的YH高频再生叶柄外植体进行了多组体分析。TDZ处理组与CK组比较，发现了494个上调的差异表达基因和293个下调的差异表达基因。GO分析表明，这些差异主要与膜的整体成分、转录因子活性和磷酸信号转导系统有关。代谢组分析鉴定了1,889种化合物，其中包括205种差异积累的代谢物(DAM)。在这些堤坝中，脂质和类脂分子的数量最多，占所有堤坝的27.8%。转录组图谱、代谢和激素综合分析表明，TDZ重组腺嘌呤类细胞分裂素(CTKs)，负调控内源CTK信号转导。此外，TDZ处理在不改变桑树生长素生物合成的情况下，通过增加生长素通量载体基因和下游调控基因的表达，增强了生长素信号转导。综上所述，本研究为桑树的体外再生提供了一种新的方法，并有助于更好地了解TDZ处理后桑树的转录和代谢变化。
Thidiazuron (TDZ) is a plant growth regulator that is widely used as a defoliant and cytokinin-like compound. Additionally, mulberry is an economically important crop. However, regeneration is difficult in most mulberry species, and the regulatory mechanism controlling the regeneration process remains unclear. In this work, an efficient in vitro shoot regeneration system for mulberry was established by TDZ application. The results showed that the optimum adventitious bud induction, 70.83% in tetraploid M. notabilis cv. Chuanbei1 (CB1) and 96.82% in M. abla cv. Yunhua (YH), was obtained by the shoot pretreatment method using petiolate leaves as explants. The high-frequency regeneration petiolate leaf explants of YH treated with TDZ (T8) and the control (CK) were subjected to multiomic analyses. The comparison of the TDZ-treated with the CK groups identified 494 upre-gulated differentially expressed genes (DEGs) and 293 downregulated DEGs. GO analysis indicated that these DEGs were related mostly to integral component of membrane, transcription factor activity, and phosphorelay signal transduction system. Metabolomic analysis identified 1,889 compounds, including 205 differentially accumulated metabolites (DAMs). Of these DAMs, the number of lipids and lipid-like molecules was highest and accounted for 27.8% of all DAMs. Transcriptome profile, metabolomic, and phytohormone integrated analyses revealed that TDZ rearranged adenine-type cytokinins (CTKs) and negatively regulated endogenous CTK signal transduction. Moreover, TDZ treatment enhanced auxin signal transduction by increasing the expression of auxin flux carrier genes and downstream regulated genes without changing auxin biosynthesis in mulberry. In sum-mary, this study provides a new method for mulberry in vitro regeneration and contributes to a better under-standing of the transcriptional and metabolic changes that occur in mulberry after TDZ treatment.