Changes in the concentration of activation factor for phosphofructokinase in hepatocytes in response to glucose and glucagon.
Changes in the concentration of activation factor for phosphofructokinase in hepatocytes in response to glucose and glucagon.
复制标题
肝细胞中磷酸果糖激酶激活因子浓度对葡萄糖和胰高血糖素的反应发生变化。
DOI：
10.1016/s0006-291x(80)80040-4
复制
发表时间：
1980
影响因子：
3.1
通讯作者：
Uyeda,K
中科院分区：
文献类型：
--
作者：
Richards,CS;Uyeda,K
最近我们发现了“激活因子”，它可以阻止 ATP 对肝脏磷酸果糖激酶的抑制（Proc. Natl. Acad. Sci.，出版中）。使用分离的肝细胞研究了胰高血糖素和葡萄糖施用对该因子水平的影响。给予 20 mM 葡萄糖后 8 分钟内，肝细胞中该因子的浓度增加至少 15 倍。给予胰高血糖素（10−7M）后，激活因子的水平迅速下降，并在 2 分钟内下降至原始浓度的 1/13。据认为，胰高血糖素通过以下方式抑制肝脏中的磷酸果糖激酶：(1)降低激活因子的水平，以及(2)酶的磷酸化。
Recently we discovered “activation factor” which prevents ATP inhibition of liver phosphofructokinase (Proc. Natl. Acad. Sci., in press). The effect of glucagon and glucose administration on the levels of the factor was investigated using isolated hepatocytes. The concentration of the factor in hepatocytes increased at least 15-fold within 8 min after administration of 20 mM glucose. Upon glucagon administration (10−7M), the level of activation factor decreased rapidly and within 2 min the amount decreased to 1/13 of the original concentration. It is proposed that glucagon acts to inhibit phosphofructokinase in the liver by (1) decreasing the level of activation factor, and (2) phosphorylation of the enzyme.