Anti-inflammatory effects of aronia extract on rat endotoxin-induced uveitis
Anti-inflammatory effects of aronia extract on rat endotoxin-induced uveitis
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DOI：
10.1167/iovs.04-0715
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发表时间：
2005-01-01
影响因子：
4.4
通讯作者：
Ohno, S
中科院分区：
文献类型：
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作者：
Ohgami, K;Ilieva, I;Ohno, S
目的。据报道，野樱莓粗提物 (ACE) 含有高含量的多酚化合物，在体外和体内具有抗氧化作用。在这项研究中，注意力集中在ACE的抗氧化作用上。本研究的目的是探讨 ACE 对大鼠内毒素诱导的葡萄膜炎 (EIU) 的影响。此外，在体外用ACE处理的小鼠巨噬细胞系(RAW 264.7)中研究内毒素诱导的诱导型一氧化氮合酶(iNOS)和环氧合酶(COX)-2蛋白的表达，以阐明其抗炎作用。方法。通过足垫注射脂多糖（LPS）在雄性Lewis大鼠中诱导EIU。 LPS接种后立即静脉注射1、10或100mg ACE或10mg泼尼松龙。 24小时后，采集双眼房水，测定房水中浸润细胞数、蛋白质浓度、一氧化氮（NO）、前列腺素（PG）-E2和TNF-α水平。将用不同浓度的ACE处理的RAW 264.7细胞与10μg/mL LPS一起孵育24小时。通过酶联免疫吸附测定测定 NO、PGE2 和 TNF-α 的水平。通过Western blot分析iNOS和COX-2蛋白的表达。结果。 ACE治疗组中炎症细胞的数量、蛋白质浓度以及房水中NO、PGE2和TNF-α的水平均以剂量依赖性方式显着降低。此外，100毫克ACE的抗炎作用与10毫克泼尼松龙一样强。 ACE 的抗炎作用比单独使用槲皮素或花青素更强。 ACE 还以剂量依赖性方式抑制体外 RAW 264.7 细胞中 LPS 诱导的 iNOS 和 COX-2 蛋白表达。结论。结果表明，ACE 具有剂量依赖性抗眼部炎症作用，这是由于直接阻断 iNOS 和 COX-2 酶的表达，从而抑制 NO、PGE2 和 TNF-α 的产生。
PURPOSE. Aronia crude extract (ACE) with high levels of polyphenol compounds has been reported to have antioxidative effects in vitro and in vivo. In this study, attention was focused on the antioxidant effect of ACE. The purpose of the present study was to investigate the effect of ACE on endotoxin-induced uveitis (EIU) in rats. In addition, the endotoxin-induced expression of the inducible nitric oxide synthase ( iNOS) and cyclooxygenase (COX)-2 proteins was investigated in a mouse macrophage cell line (RAW 264.7) treated with ACE in vitro, to clarify the anti-inflammatory effect.METHODS. EIU was induced in male Lewis rats by a footpad injection of lipopolysaccharide (LPS). Immediately after the LPS inoculation, 1, 10, or 100 mg ACE or 10 mg prednisolone was injected intravenously. After 24 hours, the aqueous humor was collected from both eyes, and the number of infiltrating cells, protein concentration, nitric oxide ( NO), prostaglandin (PG)-E2, and TNF-alpha levels in the aqueous humor were determined. RAW 264.7 cells treated with various concentrations of ACE were incubated with 10 mug/mL LPS for 24 hours. Levels of NO, PGE2, and TNF-alpha were determined by an enzyme-linked immunosorbent assay. The expression of iNOS and COX-2 proteins was analyzed by Western blot analysis.RESULTS. The number of inflammatory cells, the protein concentrations, and the levels of NO, PGE2, and TNF-alpha in the aqueous humor in the groups treated with ACE were significantly decreased in a dose-dependent manner. In addition, the anti-inflammatory effect of 100 mg ACE was as strong as that of 10 mg prednisolone. The anti-inflammatory action of ACE was stronger than that of either quercetin or anthocyanin administered alone. ACE also suppressed LPS-induced iNOS and COX-2 protein expressions in RAW 264.7 cells in vitro in a dose-dependent manner.CONCLUSIONS. The results suggest that ACE has a dose-dependent anti-ocular inflammatory effect that is due to the direct blocking of the expression of the iNOS and COX-2 enzymes and leads to the suppression of the production of NO, PGE2, and TNF-alpha.