Computer simulation of the role of protein corona in cellular delivery of nanoparticles
Computer simulation of the role of protein corona in cellular delivery of nanoparticles
复制标题
计算机模拟蛋白质电晕在纳米颗粒细胞递送中的作用
DOI：
10.1016/j.biomaterials.2014.06.033
复制
发表时间：
2014-10-01
期刊：
影响因子：
14
通讯作者：
Ma, Yu-qiang
中科院分区：
文献类型：
--
作者：
Ding, Hong-ming;Ma, Yu-qiang
了解血清蛋白在纳米粒子传递过程中的作用在生物医学中具有重要意义。通过耗散粒子动力学模拟，我们系统地研究了纳米粒子-蛋白质冠复合物与不同类型细胞膜之间的相互作用。研究发现，人血清白蛋白（HSA）只吸附在带电（特别是带正电）和疏水纳米颗粒表面。更重要的是，我们还提供了HSA吸附对纳米颗粒体内运输（即免疫反应和靶向细胞摄取）的影响的具体见解。我们的研究结果表明，蛋白质电晕可以改变疏水纳米粒子的相互作用模式，增强带电纳米粒子与巨噬细胞细胞膜的相互作用，但也可能导致疏水纳米粒子插入失败和带电纳米粒子与癌细胞细胞膜的靶向特异性丧失。这些结果有助于更好地理解蛋白质冠的生物学意义，并可能为未来药物递送纳米颗粒的更好设计提供一些有用的建议。(C) 2014 Elsevier Ltd.版权所有。
Understanding the role of serum protein in the process of nanoparticle delivery is of great importance in biomedicine. Here, by using dissipative particle dynamics simulations, we systematically investigate the interactions between the nanoparticle-protein corona complex and cell membranes of different types. It is found that the human serum albumin (HSA) will just adsorb onto charged (especially for positively charged) and hydrophobic nanoparticle surface. More importantly, we also provide specific insights into the effect of HSA adsorption on the in vivo transportation of nanoparticle (i.e., immune response and targeted cellular uptake). Our results show that the protein corona can change the interaction modes of hydrophobic nanoparticles and enhance the interaction of charged nanoparticles with macrophage cell membranes, while it may also cause the failure of insertion of hydrophobic nanoparticles and the loss of targeting specificity of charged nanopartides with cancer cell membranes. These results can help better understand the biological significance of protein corona and may give some useful suggestions on better design of future nanoparticles in drug delivery. (C) 2014 Elsevier Ltd. All rights reserved.