Claudin-1 and claudin-5 expression patterns differentiate lung squamous cell carcinomas from adenocarcinomas
Claudin-1 and claudin-5 expression patterns differentiate lung squamous cell carcinomas from adenocarcinomas
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DOI：
10.1038/modpathol.3800835
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发表时间：
2007-09-01
期刊：
影响因子：
7.5
通讯作者：
Citi, Sandra
中科院分区：
文献类型：
--
作者：
Paschoud, Serge;Bongiovanni, Massimo;Citi, Sandra
我们通过免疫组织化学和定量实时逆转录聚合酶链反应（RT-PCR）研究了紧密连接蛋白在人肺鳞状细胞癌和腺癌中的表达。我们发现肿瘤的诊断与紧密蛋白 (CLDN)-1 或 CLDN-5 的阳性之间存在统计学上显着的相关性。鳞状细胞癌和支气管上皮基底细胞的 CLDN-1 呈阳性，CLDN-5 呈阴性，而腺癌、正常圆柱细胞和肺细胞的 CLDN-5 呈阳性，而 CLDN-5 呈阴性。 CLDN-1，表明肿瘤发生和进展的不同途径。在两种类型的肿瘤中均检测到 CLDN-4 和 ZO-1 染色，而在鳞状细胞癌中未检测到 cingulin (CGN)。定量 RT-PCR 用于评估大量紧密连接蛋白转录水平的变化。在鳞状细胞癌中，我们观察到 JAM-1、occludin、CLDN-3、CLDN-4、CLDN-7、CGN、ZO-2 和 ZO-3 的 mRNA 水平显着降低，而 CLDN-1 mRNA 增加。在腺癌中，当与支气管细胞的转录水平进行比较时，我们观察到 CLDN-1、CLDN-3、CLDN-4、CLDN-7、ZO-2 和 ZO-3 的 mRNA 水平显着降低。这些结果表明，人肺肿瘤中紧密连接蛋白表达的表征可以成为一种额外的诊断工具，并为其组织发生提供新的见解。
We investigated the expression of tight junction proteins in human lung squamous cell carcinomas and adenocarcinomas by immunohistochemistry and quantitative real-time reverse transcription-polymerase chain reaction ( RT-PCR). We found a statistically significant correlation between diagnosis and positivity of tumors with either claudin ( CLDN)- 1 or CLDN- 5. Squamous cell carcinomas and basal cells of bronchial epithelium were positive for CLDN- 1 and negative for CLDN- 5, whereas adenocarcinomas, normal cylindrical cells and pneumocytes were positive for CLDN- 5 and negative for CLDN- 1, suggesting different pathways in tumor development and progression. CLDN- 4 and ZO-1 staining were detected in both types of tumors, whereas cingulin ( CGN) was not detected in squamous cell carcinomas. Quantitative RT-PCR was used to evaluate changes in transcript levels for a large panel of tight junction proteins. In squamous cell carcinomas, we observed statistically significant decreases in the mRNA levels of JAM- 1, occludin, CLDN- 3, CLDN- 4, CLDN- 7, CGN, ZO-2 and ZO-3, and an increase in CLDN- 1 mRNA. In adenocarcinomas, when transcript levels were compared with bronchial cells, we observed statistically significant decreases in the mRNA levels of CLDN- 1, CLDN- 3, CLDN- 4, CLDN- 7, ZO-2 and ZO-3. These results indicate that characterization of tight junction protein expression in human lung tumors can be an additional diagnostic tool and provide new insights on their histogenesis.