Understanding the gut-kidney axis among biopsy-proven diabetic nephropathy, type 2 diabetes mellitus and healthy controls: an analysis of the gut microbiota composition
Understanding the gut-kidney axis among biopsy-proven diabetic nephropathy, type 2 diabetes mellitus and healthy controls: an analysis of the gut microbiota composition
复制标题
了解活检证实的糖尿病肾病、2 型糖尿病和健康对照之间的肠肾轴：肠道微生物群组成分析
DOI：
10.1007/s00592-019-01316-7
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发表时间：
2019-05-01
影响因子：
3.8
通讯作者：
Fu, Ping
中科院分区：
文献类型：
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作者：
Tao, Sibei;Li, Lingzhi;Fu, Ping
目的：2型糖尿病（T2DM）的患病率不断上升，肠道微生物群的参与越来越被认识到。糖尿病肾病（DN）是T2DM的主要并发症。该研究的目的是通过分析活检证实的DN，无肾脏疾病的T2DM和健康对照的肠道微生物群组成来了解肠肾轴。方法收集14例糖尿病患者、14例年龄/性别匹配的无肾脏疾病t2dm患者（DM）、14例年龄/性别匹配的健康对照（HC）和DM组家庭接触者（HH）的粪便样本。采用16sRNA微生物谱法分析菌群组成。结果DM组与HC组、DN组与DM组在肠道菌群丰富度和菌群变异方面存在显著差异。g_Prevotella_9属变量（AUC=0.9）可以准确区分DM与年龄/性别匹配的健康对照，g_eschericia - shigella和g_Prevotella_9属变量（AUC= 0.86）可以准确区分DN患者与年龄/性别匹配的DM。HH组的菌群组成与HC组接近，与DM组不同。在相同日粮条件下，同一属对DM的检测更为准确（g_Prevotella_9， AUC=0.92）。结论肠道菌群组成与活检证实的DM型DN的发生有关，通过检测粪便中g_Prevotella_9的水平可将DM与HC区分，而g_Escherichia-Shigella和g_Prevotella_9的水平可将DN与DM区分，这可能有助于DM型DN的生理病理诊断。
AimsType 2 diabetes mellitus (T2DM) has a rising prevalence and gut microbiota involvement is increasingly recognized. Diabetic nephropathy (DN) is a major complication of T2DM. The aim of the study was to understand the gut-kidney axis by an analysis of gut microbiota composition among biopsy-proven DN, T2DM without kidney disease, and healthy control.MethodsFecal samples were collected from 14 DNs, 14 age/gender-matched T2DMs without renal diseases (DM), 14 age and gender-matched healthy controls (HC) and household contacts (HH) of DM group. The microbiota composition was analyzed by 16sRNA microbial profiling approach.ResultsSubstantial differences were found in the richness of gut microbiota and the variation of bacteria population in DM compared to HC, and DN compared to DM, respectively. DM could be accurately distinguished from age/gender-matched healthy controls by the variable of genus g_Prevotella_9 (AUC=0.9), and DN patients could be accurately distinguished from age/gender-matched DM by the variables of two genera (g_Escherichia-Shigella and g_Prevotella_9, AUC=0.86). The microbiota composition of HH group was close to that of HC group, and was different from DM group. Under the same diet, DM could be more accurately detected by the same genus (g_Prevotella_9, AUC=0.92).ConclusionGut microbiota composition was explored to be related to the occurrence of biopsy-proven DN from DM. DM could be distinguished from HC by detecting g_Prevotella_9 level in feces, while DN was different from DM by the variables of g_Escherichia-Shigella and g_Prevotella_9, which potentially contributed to the physiopathological diagnosis of DN from DM.