Discrimination of human leukemia subtypes by flow cytometric analysis of cellular DNA and RNA.
Discrimination of human leukemia subtypes by flow cytometric analysis of cellular DNA and RNA.
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通过细胞 DNA 和 RNA 的流式细胞术分析区分人类白血病亚型。
DOI：
10.1182/blood.v55.2.282.bloodjournal552282
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发表时间：
1980
期刊：
影响因子：
20.3
通讯作者：
Myron R. Melamed
中科院分区：
文献类型：
--
作者：
M. Andreeff;Zbigniew Darzynkiewicz;T. Sharpless;Bayard Clarkson;Myron R. Melamed
新开发的流式细胞术技术使用吖啶橙作为荧光异染染料同时测量单个细胞的三个特征——DNA、RNA和核直径，已应用于细胞周期分析。 DNA干系测定，并对102例成人人类白血病进行分类。急性淋巴细胞白血病（L1-2）的特点是G0/1细胞的RNA较正常淋巴细胞中度增加；急性非淋巴细胞白血病 (M 1-5) 由 G0/G1 细胞的 RNA 非常高引起。两者都具有二倍体或非整倍体 DNA 茎系。慢性淋巴细胞白血病表现出二倍体 DNA、极低的增殖和低 RNA，与使用发现的正常 B 细胞的典型特征相似。在慢性粒细胞白血病中，区分出两种细胞群，一种具有高RNA，另一种具有非常低的RNA，并且由于多形核白细胞的细胞核剥离、展开而导致核直径延长。通过非整倍体 DNA 值鉴定的白血病母细胞的数量与传统显微镜计数密切相关，并且可以在治疗过程中进行跟踪。因此，吖啶橙流式细胞术可用于区分人类白血病的亚型，确定细胞周期阶段，以及检测和监测非整倍体白血病干系。
A newly developed flow cytometry technique for simultaneous measurements of three features of individual cells--DNA, RNA, and nuclear diameter--using acridine orange as a fluorescent metachromatic dye, has been applied to cell-cycle analysis. DNA stemline determination, and to classification of 102 cases of human leukemias in adults. Acute lymphoblastic leukemia (L1-2) was characterized by moderately increased RNA of G0/1 cells as compared to normal lymphocytes; acute nonlymphoblastic leukemia (M 1-5) by very high RNA of G0/G1 cells. Both had either diploid or aneuploid DNA stemlines. Chronic lymphocytic leukemia showed diploid DNA, very low proliferation, and low RNA, similar to that found by use to be typical for normal B cells. In chronic myelogenous leukemia, two cell populations were distinguished, one with high RNA, the other with very low RNA and elongated nuclear diameter due to stripped, unfolded nuclei of polymorphonuclear leukocytes. The number of leukemic blast cells, identified by aneuploid DNA values, correlates well with conventional microscopy counts and could be followed during the course of treatment. Thus, acridine orange flow cytometry can be used to discriminate subtypes of human leukemias, to determine cell cycle stages, and to detect and monitor aneuploid leukemia stemlines.