The centrosomal protein Lats2 is a phosphorylation target of Aurora-A kinase
The centrosomal protein Lats2 is a phosphorylation target of Aurora-A kinase
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DOI：
10.1111/j.1356-9597.2004.00732.x
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发表时间：
2004-05-01
期刊：
影响因子：
2.1
通讯作者：
Nojima, H
中科院分区：
文献类型：
--
作者：
Toji, S;Yabuta, N;Nojima, H
人Lats 2是一种新的丝氨酸/苏氨酸激酶，是包括果蝇肿瘤抑制因子lats/warts的Lats激酶家族的成员。Lats 1是Lats 2的对应物，在有丝分裂中被磷酸化并定位于有丝分裂器。然而，Lats 2的调节、功能和细胞内分布仍不清楚。在这里，我们表明Lats 2是Aurora-A激酶的一个新的磷酸化靶点。我们首先在体外证明了Lats 2的磷酸化残基是S83。识别这种磷酸化的S83的抗体表明磷酸化也在体内发生。我们发现Lats 2与Aurora-A短暂相互作用，并且Lats 2和Aurora-A在细胞周期期间共定位于中心体。此外，我们发现Aurora-A诱导的S83磷酸化对Lats 2的抑制部分扰乱了其中心体定位。基于这些观察，我们得出结论，S83的Lats 2是极光-A的磷酸化的目标，这种磷酸化起着作用的中心体定位的Lats 2。
Human Lats2, a novel serine/threonine kinase, is a member of the Lats kinase family that includes the Drosophila tumour suppressor lats/warts. Lats1, a counterpart of Lats2, is phosphorylated in mitosis and localized to the mitotic apparatus. However, the regulation, function and intracellular distribution of Lats2 remain unclear. Here, we show that Lats2 is a novel phosphorylation target of Aurora-A kinase. We first showed that the phosphorylated residue of Lats2 is S83 in vitro. Antibody that recognizes this phosphorylated S83 indicated that the phosphorylation also occurs in vivo. We found that Lats2 transiently interacts with Aurora-A, and that Lats2 and Aurora-A co-localize at the centrosomes during the cell cycle. Furthermore, we showed that the inhibition of Aurora-A-induced phosphorylation of S83 on Lats2 partially perturbed its centrosomal localization. On the basis of these observations, we conclude that S83 of Lats2 is a phosphorylation target of Aurora-A and this phosphorylation plays a role of the centrosomal localization of Lats2.