Characterization of Salmonella enterica subspecies I genovars by use of microarrays
Characterization of Salmonella enterica subspecies I genovars by use of microarrays
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DOI：
10.1128/jb.186.17.5883-5898.2004
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发表时间：
2004-09-01
影响因子：
3.2
通讯作者：
McClelland, M
中科院分区：
文献类型：
--
作者：
Porwollik, S;Boyd, EF;McClelland, M
肠沙门氏菌亚种1几乎是所有温血动物感染沙门氏菌的罪魁祸首。在亚种1中有超过2300种已知的血清型，它们的流行程度和在不同宿主中引起的疾病有所不同。这些血清型中只有少数会导致人类和家畜中的大多数沙门氏菌感染。最流行的79株血清型的基因含量；基因芯片分析。同一血清型内的菌株往往因数百个基因的存在和缺失而有所不同。基因含量有时在同一血清型内比在不同血清型之间差异更大。具有不同基因内容的菌株群可称为“基因型”，以区别于血清型。通过基因型分型检测到沙门氏菌参考B集合中的若干错配，并随后进行血清学确认。正如血清学已被证明有助于了解沙门氏菌的宿主范围和致病表现一样，基因型也可能进一步确定以前未被认识到的沙门氏菌感染的具体特征。
Subspecies 1 of Salmonella enterica is responsible for almost all Salmonella infections of warm-blooded animals. Within subspecies 1 there are over 2,300 known serovars that differ in their prevalence and the diseases that they cause in different hosts. Only a few of these serovars are responsible for most Salmonella infections in humans and domestic animals. The gene contents of 79 strains from the most prevalent serovars; were profiled by microarray analysis. Strains within the same serovar often differed by the presence and absence of hundreds of genes. Gene contents sometimes differed more within a serovar than between serovars. Groups of strains that share a distinct profile of gene content can be referred to as "genovars" to distinguish them from serovars. Several misassignments within the Salmonella reference B collection were detected by genovar typing and were subsequently confirmed serologically. Just as serology has proved useful for understanding the host range and pathogenic manifestations of Salmonella, genovars are likely to further define previously unrecognized specific features of Salmonella infections.