Cutting artefacts and cutting process in vitreous sections for cryo-electron microscopy
Cutting artefacts and cutting process in vitreous sections for cryo-electron microscopy
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DOI：
10.1016/j.jsb.2005.01.003
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发表时间：
2005-04-01
影响因子：
3
通讯作者：
Dubochet, J
中科院分区：
文献类型：
--
作者：
Al-Amoudi, A;Studer, D;Dubochet, J
玻璃体切片冷冻电子显微镜（CEMOVIS）最近已被证明可以提供具有前所未有的质量和分辨率的生物标本图像。然而，切割部分仍然是主要的困难。在这里，我们检查影响切片质量的参数，并分析由此产生的伪影。它们特别是：刀痕，压缩，裂缝和颤振。我们提出了一个模型，考虑到粘性流和裂缝之间的相互作用。我们确认，裂缝只形成在一侧的部分，并定义的条件下，他们可以避免。颤振是由于刀刃部分的摩擦引起的不规则压缩的影响，也探讨了防止这种情况的条件。在没有裂缝和颤振的情况下，截面的大部分被近似均匀地压缩。在这种近似中，可以通过对截面的大部分进行简单的线性变换来校正压缩。提出了一个研究计划，以测试和完善我们的理解的切片过程。(c)2005年爱思唯尔公司All rights reserved.
Cryo-electron microscopy of vitreous sections (CEMOVIS) has recently been shown to provide images of biological specimens with unprecedented quality and resolution. Cutting the sections remains however the major difficulty. Here, we examine the parameters influencing the quality of the sections and analyse the resulting artefacts. They are in particular: knife marks, compression, crevasses, and chatter. We propose a model taking into account the interplay between viscous flow and fracture. We confirm that crevasses are formed on only one side of the section, and define conditions by which they can be avoided. Chatter is an effect of irregular compression due to friction of the section of the knife edge and conditions to prevent this are also explored. In absence of crevasses and chatter, the bulk of the section is compressed approximately homogeneously. Within this approximation, it is possible to correct for compression by a simple linear transformation for the bulk of the section. A research program is proposed to test and refine our understanding of the sectioning process. (c) 2005 Elsevier Inc. All rights reserved.